Callosobruchus chinensis L. is an important pest on mung beans. Alternative control of C. chinensis using botanical insecticides from extracts of Jatropha curcas and Cymbopogon nardus. The purpose of the research was to compare the toxicity of extracts and determine their compatibility. Toxicity testing methods use to contact, fumigation, and seed dressing methods. The research data was tested by probit analysis. The results showed that the toxicity of the contact method extract was more toxic than fumigation and seed dressing. The highest LC50 and LC95 values for the C. nardus extract applied with the contact method were observed at 72 HAT at 0.17% and 0.40% more toxic than the J. curcas extract at 0.21% and 2.14%. The best mixed extract of J. curcas and C. nardus was at a ratio of 2:1 with strong synergistic interaction (LC50) and weak synergy (LC95)

ardiansyah, sigit

Nuryanti, Ni Siluh Putu

Wahyudi, Anung

English

UIN (Universitas Islam Negeri) Walisongo Journals

Al-Hayat: Journal of Biology and Applied Biology Volume 5, No 2 (2022): 139-144 DOI. 10.21580/ah.v5i2.12503 139 Volume 5, No 2 (2022) |  Toxicity of Jatropha curcas and Cymbopogan nardus Extracts Against Pests Callosobruchus chinensis on Mung Beans  Sigit Ardiansyah1*, Ni Siluh Putu Nuryanti2, Anung Wahyudi3 1Postgraduate Students of Applied Masters of Food Security  2,3Lecturer of Applied Masters of Food Security  Abstract Callosobruchus chinensis L. is an important pest on mung beans. Alternative control of C. chinensis using botanical insecticides from extracts of Jatropha curcas and Cymbopogon nardus. The purpose of the research was to compare the toxicity of extracts and determine their compatibility. Toxicity testing methods use to contact, fumigation, and seed dressing methods. The research data was tested by probit analysis. The results showed that the toxicity of the contact method extract was more toxic than fumigation and seed dressing. The highest LC50 and LC95 values for the C. nardus extract applied with the contact method were observed at 72 HAT at 0.17% and 0.40% more toxic than the J. curcas extract at 0.21% and 2.14%. The best mixed extract of J. curcas and C. nardus was at a ratio of 2:1 with strong synergistic interaction (LC50) and weak synergy (LC95). Keywords: botanical insecticides; callosobruchus chinensis; cymbopogon nardus; jatropha curcas  Introduction Mung beans are a species of legume that is widely used in Indonesia as a protein and mineral source. Mung beans, on the other hand, are a legume that can be attacked by pests after harvest and during storage. According to (Nurbaekah, Sumadi, & Nuraini, 2018) , Callosobruchus chinensis L. is the most common post-harvest pest that damages mung beans, with losses of up to 70%. As a result, mung beans aren't very useful. Chemical pesticides are commonly used to manage pests, but this practice can contaminate stored mung beans with harmful chemical residues. As a result, it is vital to obtain safer and more environmentally friendly insecticides, such as botanical insecticides. Herbivore rejection, prevention, toxicity, and growth inhibition are all possible with botanical pesticides produced from secondary metabolites (Anggraito et al., 2018). Indonesia, which is located in the tropics, has a diverse flora that can be exploited as a source of botanical pesticide raw materials. According to (Saenong, 2016), over 400 thousand different varieties of chemical plants have been found in Indonesia, with 10,000 of them containing secondary metabolites. Cymbopogon nardus and Jatropha curcas are examples of plants that produce secondary metabolites. J. curcas and C. nardus have a lot of potential as insecticides. According to (Devappa et al., 2012), the phorbol ester compound from J. curcas oil has a contact toxic action and decreases the feeding activity of Spodoptera frugiferda larvae. The leaf powder of J. curcas protects bambara beans against Callosobruchus subinnotatus (Dattijo et al., 2018). According to (Hasan, Harahap, & Hidayat, 2018), C. nardus is Sigit Ardiansyah, Ni Siluh Putu Nuryanti, Anung Wahyudi 140 | Volume 5, No 2 (2022) poisonous to Callosobruchus maculatus. C. nardus oil contains monoterpenes such as limonene, citronellal, and geraniol, which can help plants defend themselves against pests (Maryani, Dono, & Yulia, 2019). The toxicity of botanical pesticides derived from these two plants to C. chinensis is still limited. The purpose of the research was to compare the toxicity of extracts of J. curcas and C. nardus on several application methods to control C. chinensis Research Methods  Insect Culture The C. chinensis population used was from Plant Laboratory 1 at Lampung State Polytechnic. Five pairs of Imago C. chinensis were inserted into a container holding mung bean seeds to breed. C. chinensis was bred to create a novel C. chinensis imago. The emergent imago was utilized as a test insect. Extraction The pressing process was used to extract J. curcas. A grinding machine was used to process dried J. curcas seeds. It was then heated for 10 minutes at 100 °C before being pressed using a hydraulic press until the oil was extracted. The distillation process was used to extract C. nardus. C. nardus was chopped, then air-dried for three days before being distilled via distillation. Distillation is carried out for 6 hours at 130 °C, until no oil droplets remain. The oil is left for a while after distillation to ensure that the water and oil are thoroughly separated  Emulsification Formulation The formulation of emulsification using a low-energy emulsification method with phase inversion. The extracts of J. curcas and Tween 80 in a ratio of 1:1 were homogenized and then added with distilled water little by little to a volume of 100 ml, accompanied by stirring using a magnetic stirrer at a speed of 730 rpm at room temperature (Nuryanti et al., 2018). In the emulsification of the formulation of C. nardus extract, using the same procedure as in the emulsification of the formulation of J. curcas. Toxicity Testing of Extracts Against C. chinensis This preliminary test was conducted to determine the value of lethal concentration (LC). In this test, each extract was tested against C. chinensis by the contact application method, fumigation, and seed dressing at concentrations of 0.125, 0.25, 0.5, 1, and 2%. Observations in this test were made by taking C. chinensis mortality data with observation times of 24, 48, and 72 hours. The research data was tested by probit analysis (Finney, 1982). Follow-up Test, To estimate the concentration utilized in the follow-up test, five concentrations of each type of extract were made based on the LC50 and LC95 values from the preliminary test probit data. The method of application is the same as that employed in the preliminary test. Research Results and Discussion The results showed that the concentration of treatment with extracts of J. curcas and C. nardus tested by contact, fumigation, and seed dressing methods had a toxic effect on imago C. chinensis (Table 1, 2). The lower the value of lectal concentration (LC), the more the extract has a higher toxicity. The toxicity of the extract of J. curcas which was applied by the contact method was higher than the toxicity of the extract which was applied by the method of fumigation and seed dressing. The values of LC50 and LC95 in the extract of J. curcas which were applied using the contact method were highest at the observation 72 hours after Toxicity Of Jatropha curcas…. Volume 5, No 2 (2022) | 141 treatment (HAT) of 0.21% and 2.14%. The fumigation method was 1.29% and 54.31%, while the seed dressing method was 0.32% and 30.50%. This indicates that the extract of J. curcas applied by the contact method has the most toxic effect compared to other methods Table 1 Estimation of probit analysis parameters of Jatropha curcas and Cymbopogon nardus extracts against Callosobruchus chinensis by several methods at LC50 Methods Lethal Consentration HATa J. curcas (%) C. nardus (%) Contact LC50 24 0,53 0,21 LC50 48 0,34 0,18 LC50 72 0,21 0,17 Fumigation LC50 24 6,43 2,93 LC50 48 2,90 1,57 LC50 72 1,29 0,92 Seed dreassing LC50 24 4,80 4,66 LC50 48 1,60 1,82 LC50 72 0,32 0,61 Note: HAT = Hours After Treatment Table 2 Estimation of probit analysis parameters of Jatropha curcas and Cymbopogon nardus extracts against Callosobruchus chinensis by several methods at LC95 Methods Lethal Consentration HATa J. curcas (%) C. nardus (%) Contact LC95 24 3,51 0,53 LC95 48 2,13 0,39 LC95 72 2,14 0,40 Fumigation LC95 24 130,72 70,94 LC95 48 92,81 34,12 LC95 72 54,31 9,66 Seed dreassing LC95 24 254,62 350,83 LC95 48 143,29 116,14 LC95 72 30,50 13,65 Note: HAT = Hours After Treatment  J. curcas extract contains secondary metabolite compounds including curcin, lectin, phorbol ester, esterase, and lipase (Asmanizar et al., 2020). Phorbol ester and curcin are the main chemical compounds found in J. curcas which work as contact poison, stomach poison, and nerve poison (Banjarnahor et al., 2016). The mechanism of the chemical content enters the digestive tract of pests and interferes with metabolic processes in the body of pests. So that the pests experience paralysis, no appetite, and eventually die. The highest LC50 and LC95 values in the C. nardus extract applied with the contact method were observed at 72 HAT of 0.17% and 0.40%, fumigation of 0.92% and 9.66%, seed dreassing of 0.61 and 13.65%. Based on Sigit Ardiansyah, Ni Siluh Putu Nuryanti, Anung Wahyudi 142 | Volume 5, No 2 (2022) the LC values, it shows that insecticides from C. nardus extract which were applied by the contact method had a higher toxicity effect than other methods. In addition, the toxicity test of the C. nardus extract applied by contact method was more toxic than the J. curcas extract because the LC50 and LC95 of the C. nardus extract were smaller than that of the J. curcas extract. C. nardus extract had the ability to kill Crocidolomia pavonana larvae (kontambunan, Salaki, & Tarore, 2019)  [12]. In addition, C. nardus can control plant lice, insects (Sitophilus sp, Callosobruchus sp.) and nematodes (fahrudin, 2018)  [13]. C. nardus oil has an LC50 of 0.36% against Mizus persicae (Maryani, Dono, & Yulia, 2019) . Citronella is the most abundant chemical in C. nardus (kontambunan, Salaki, & Tarore, 2019). Insecticides containing citronella as an active ingredient are classed as contact poisons and stomach poisons (Una & Wahyuni, 2019) [14]. Insecticides classed as contact poisons enter the target insect's body through the cuticle, trachea, sensory glands, and other organs linked to the cuticle. Citronella acts by inhibiting the enzyme acetyl cholinesterase, causing the amino acid serine in the enzyme's asteratic core to be phosphorylated. The accumulation of acetylcholine causes poisoning symptoms, which include motor nervous system abnormalities, convulsions, and respiratory paralysis, all of which lead to death (Kamelia et al., 2020). Conclusion The insecticide toxicity of  J. curcas and C. Nardus extracts to the imago of C. chinensis which was applied by the contact method was more toxic than the extracts which were applied by the fumigation and seed dreassing methods. The highest LC50 and LC95 values for the C. nardus extract applied with the contact method were observed at 72 HAT, namely 0.17% and 0.40% more toxic than the J. curcas extract, which were 0.21% and 2.14%.   Acknowledgments Thank you to Lampung State Polytechnic for provide facilities and equipment in this research, as well as all parties who helped in this research.  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Toxicity Of Jatropha curcas and Cymbopogon nardus Extracts Against Pests Callosobruchus chinensis On Mung Beans

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Toxicity Of Jatropha curcas and Cymbopogon nardus Extracts Against Pests Callosobruchus chinensis On Mung Beans

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