High concentration of nitrate (NO3) in water resources has become a widespread and important environmental contaminant, being anthropogenic nitrogen input the principal source of NO3− pollution (Arauzo, 2017). Underanaerobic conditions, microbial reduction of NO3 to N2(g) to oxidize dissolved organic carbon (DOC) is the principal NO3 attenuation process in groundwater aquifers (Matchett et al., 2019)