Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166This article summarizes the configurations involving isotope ratio mass spectrometry (IRMS) technology available at the CCiTUB and the wide range of possible applications. Some examples of these applications are shown

Marimon Corbella, Rosa Maria

Perona Moreno, Joaquim

Teixidor Casamitjana, Pilar

English

Diposit Digital de la Universitat de Barcelona

 ChT.8 Isotope Ratio - Mass SpectrometryRosa Maria Marimon1, Joaquim Perona1, and Pilar Teixidor2 1 Unitat de Medi Ambient, CCiTUB. Facultat de Geologia. Martí Franquès, s/n. 08028 Barcelona, Spain. 2 Unitat de Cromatografia de Gasos i Espectrometria de Masses. CCiTUB, Lluís Solé i Sabarís 1-3. 08028 Barcelona, Spain. email: marimon@ccit.ub.edu, jperona@ccit.ub.edu, teixidor@ccit.ub.edu Abstract. This article summarizes the configurations involving isotope ratio mass spectrometry (IRMS) technology available at the CCiTUB and the wide range of possible applications. Some examples of these applications are shown. Handbook of instrumental techniques from CCiTUBIsotope Ratio - Mass Spectrometry  1 ChT.8 1.  Introduction The natural abundances of stable isotopes for any given element are not constant in nature due to a great variety of physical and chemical fractionation processes that constantly occur. The isotope ratio mass spectrometry (IRMS) is a technology that allows us to measure these small variations in isotopic abundances of the major light elements in nature (H, C, N, O, S) with high precision. 2.  Methodology An isotope ratio mass spectrometer consists of three main parts: • Electronic ionization source (high stability and yield of ionization) • Magnetic field analyzer (effective separation of different ion beams) • Multiple Faraday cups detector (robust collection of the specific ions)  Gas sample introduction can be performed directly by means of dual inlet (DI) systems or via an additional hyphenated technique, referred to as continuous flow (CF) systems, through a carrier gas, e.g. chromatography, elemental analysis, pyrolysis. Both systems are available at the Scientific and Technological Centers of the University of Barcelona, comprising several isotope ratio mass spectrometers with different sample preparation devices. These peripherals are carbonate device, equilibration-GC system (gas bench, GB), elemental analyzers (EA), pyrolyzers (TC/EA), gas chromatographs (GC) and liquid chromatograph (LC).  Such variety of configurations allows a full range of applications in different areas:  • Life and Earth sciences (physiology, ecology, hydrology, climate, marine geosciences, biogeochemistry) • Health sciences (biochemistry, metabolism, physiology, nutrition) • Forensic science • Archaeology  The different applications comprise the analysis of samples of natural isotopic composition and low level of enrichment (up to 2-3% heavy isotope), where isotopes can be used as tracers of metabolic and fluxomic pathways. In our laboratories we have implemented several routine measurements for specific type of samples, covering inorganic and organic fields.  The stable isotope analyses that we perform in our laboratories are shown in Table 1. 3.  Examples of applications 3.1.  DI-IRMS. Carbonate analysis in foraminifera. Foraminifera have been distributed widely in the ocean from the Cambrian period to the present. Their hard shells, mainly consisting in CaCO3, are often preserved within seafloor sediment as microfossils. The analysis of their stable carbon and oxygen isotopic compositions (δ13C and δ18O) is useful because these parameters are paleoindicators of the paleoenvironment at the time when they were alive. To analyse the stable isotopic composition of foraminiferal shells, more than 20 micrograms of CaCO3 are needed. In order to obtain a reliable result, the weight of samples and reference materials should be similar. This is why samples are weighted prior to be transferred to a reaction tube, which is placed in the automatic carbonate device feed system. The carbonate device works according to the McCrea method (McCrea, 1950). This method releases CO2 from the CaCO3 and the gas flows into the IRMS (see Fig. 1).  Isotope Ratio - Mass Spectrometry  2 ChT.8 Table 1. Stable isotope analyses performed at the CCiTUB by using different IRMS configurations   Figure 1. Example of isotope records in an oceanic core (Ferretti et al, 2010).  Technique δ13C δD δ15N δ18O δ34S DI-IRMS Carbonate microsamples   Carbonate microsamples  GB-IRMS CO2 from air Water samples  Water samples   DIC in water samples CO2 from air EA-IRMS  TC/EA-IRMS Solid and liquid bulk material Solid and liquid bulk material Solid and liquid bulk material Solid and liquid bulk material Solid and liquid bulk material GC-IRMS Specific Organic compounds (VOCs and SVOCs) Specific Organic compounds (VOCs and SVOCs) Nitrogenated organic compounds   LC-IRMS Water soluble organic compounds (total extract and compound specific)     Isotope Ratio - Mass Spectrometry  3 ChT.8 3.2.  CF-IRMS At our IRMS facilities, several methodologies have been developed in CF-IRMS. Some of them are focused to environmental applications as the stable isotopes signatures contribute to the knowledge of the origin and fate of specific pollutants found in different areas. Moreover, the isotopic analyses contribute to control the natural or induced degradation processes (remediation). Other applications are related to metabolism and physiology, where stable isotopes are used as tracers of different processes. 3.2.1.  δ15N and δ18O of dissolved nitrates in waters . The analysis of the isotopic composition of the ions involved in the denitrification reactions allow us to determine which processes control the natural attenuation (Otero et al., 2007). In this example sample preparation is as follows. For the δ15NNO3 and δ18ONO3 analysis, dissolved nitrates are concentrated using anion-exchange columns, after extracting the sulphates and phosphates by precipitation. Afterwards, dissolved nitrates are eluted with HCl and converted to AgNO3 by adding silver oxide. The liophilised AgNO3 is enclosed in a Sn and/or Ag capsule for the subsequent δ15N and/or δ18O analysis by EA-IRMS and/or EA/TC-IRMS. The isotopic values δ15N vs. δ18O of dissolved NO3- found in different sampling sites of a regional area near Barcelona (Spain) are shown in Fig.2. The isotopic composition of nitrates indicates that dissolved nitrate is mainly derived from pig manure ammonium which is nitrified at the aerobic zone. It is worth noting that the δ18ONO3 indicates that nitrate is derived from fertiliser NH4, which has been nitrified. There is no evidence of the contribution of “direct” nitrate from fertilisers. Samples show a coupled increase in δ15N and δ18ONO3, with a slope 2:1. This correlation indicates the existence of denitrification processes, in almost one third of the samples. Figure 2. Isotopic values δ15N vs. δ18O of dissolved NO3- found in different sampling sites of a regional area near Barcelona (Spain). . Arrows indicate the isotopic changes linked to the different processes. 3.2.2.  Compound specific carbon isotope analysis of volatile organic compounds Using head space-solid phase microextraction (CAR-PDMS fiber) as a preconcentration technique, it is possible to detect trace levels (LOQ <10 ug/l) of halogenated and non-halogenated volatile compounds and analyze its isotopic composition with optimum precision (J.Palau et al., 2007) FertilisersNO3Nitrification ofpig manure NH4Nitificationof fertilisersNH4± Volatilization± DenitrificationMix with fertilisers NO305101520250 5 10 15 20 25 30 35 40δ 15N - NO3 (0/00)δδ δδ18 O -NO3(0 / 00)Zone 1 Zone 2 Zone 3δδ δδ18 O -NO3(0 / 00)δδ δδ18 O -NO3(0 / 00)Isotope Ratio - Mass Spectrometry  4 ChT.8 time (days)0 2 4 6 8 10 12 14concentration ( µg/L)05000100001500020000δ13 C (‰)-30-20-100108/1 2/1 0,5/1 NaOH/S2O82- molar ratiosAs in-situ chemical oxidation has emerged as an effective and low-cost method for contaminated groundwater remediation, analysis of the variation of the isotopic composition of the halogenated contaminants during different oxidation conditions was performed in order to evaluate them as a potential tool to monitor the performance of the oxidative processes applied. The observed trend in the isotopic values of δ13C allowed the quantification of the enrichment factor using Rayleigh equation (see Figs. 3 and 4).   Figure 3. Concentration (black symbols) and δ13C values (white symbols) of TCA for different NaOH/S2O82- molar ratios (Marchesi et al., 2009).   Figure 4. Concentration (white symbols) and d13C values (black symbols) of PCE for different S2O82-/Fe2+/PCE molar ratios (Marchesi et al., 2009)       mL Fe2+ added0 1 2 3 4 5 6concentration mg/L0102030405060δ13  C-35-30-25-20-15-10-5Isotope Ratio - Mass Spectrometry  5 ChT.8 3.2.3.  Study of 13C and 15N isotopic labeling on amino acids in plants by GC/C/IRMS  We have developed a methodology that allows the detection and quantification of both unlabelled and isotopically labeled amino acids so that it can be applied in a broad range of metabolomic and fluxomic studies in which stables isotopes can be used as a tracers (G. Molero et al., 2011). Typical results are shown in Figs. 5 and 6.  Figure 5. GC/C/IRMS chromatogram obtained from a mixture of aminoacids (MTBDMS-derivatives) on a PT-5 column  Figure 7. Comparison between δ15N values obtained by EA-IRMS and GC-C-IRMS y = 1,0374x - 1,0064R2 = 0,9984-50,000,0050,00100,00150,00200,00250,00300,00350,00-50,0 0,0 50,0 100,0 150,0 200,0 250,0 300,0 350,0δ15N EA-IRMSδδ δδ15 N GC-C-IRMSy = 0.9918x - 0.152R2 = 0.9925-10.000.0010.0020.0030.0040.0050.00-10.0 0.0 10.0 20.0 30.0 40.0 50.0δ15N EA-IRMSδδ δδ15 N GC-C-IRMSIsotope Ratio - Mass Spectrometry  6 ChT.8 A good linearity correlation (r2> 0,99) was observed between δ15N signature values (n= 28) obtained by EA-IRMS vs. GC-C-IRMS of four amino acids (Ala, Phe, Asp, Glu), ranging from natural to enriched (up to 300 per mil) isotopic composition. As a result, the developed methodology is applicable for natural abundance as well as for enriched materials.  3.2.4.  Analysis of δ13C in water soluble compounds by FIA-IRMS and LC-IRMS The flow injection analysis FIA-IRMS technology allows us to  analyse fast the bulk carbon isotopic composition of very small water soluble samples (50ul, 100ngC) without the complex sample preparation required for EA-IRMS analysis (see Figs. 8).  Figure 8. Comparison between δ13C values (water soluble compounds from plants) obtained by EA-IRMS and FIA-IRMS   The methodology has been applied to assess the performance of plants exposed to contrasted conditions of depleted CO2, temperature and water availability (.Aranjuelo,  2011). The technology is useful as a fast screening method for metabolic response as the recently assimilated compounds are measured (Fig. 9). Figure 9. The δ13C values obtained from WSC are less 13C depleted in comparison to those obtained from total organic matter by EA-IRMS, which reflect better the stressful conditions applied.  δ13Cy = 1,062x + 2,690R2 = 0,969-25,00-20,00-15,00-10,00-5,000,005,0010,0015,00-30,00 -25,00 -20,00 -15,00 -10,00 -5,00 0,00 5,00solid (EA-IRMS)liquid (LC-IRMS)Isotope Ratio - Mass Spectrometry  7 ChT.8 References • Aranjuelo I, 2011 (unpublished). • Ferreti et al 2010 Patrizia Ferretti, Simon J. Crowhurst, Michael A. Hall, Isabel Cacho. • (2010): North Atlantic millennial-scale climate variability 910 to 790 ka and the role of the equatorial insolation forcing. Earth and  Planetary Science Letters, 293, 28–41. • Marchesi et al., 2009. 8th International Symposium on Applied Isotope Geochemistry. • J.M. McCrea (1950): On the Isotopic Chemistry of Carbonates and a  Paleotemperature Scale. The Journal of Chemical Physics, vol.18, n.6,  p.849-857. • G. Molero, et al., 2011 Rapid Commun. Mass Spectrom. 2011, 25.599-607. • Otero et al. 2007 Multi-isotopic methods applied to monitoring groundwater nitrate attenuation in a regional system, in Towards a better efficiency in N use, Bosch Eds. 446.471. • J. Palau et al., 2007, J. Chromatogr. A, 1163:260-268. 

(unpublished). · Ferreti et al

Multi-isotopic methods applied to monitoring groundwater nitrate attenuation in a regional system, in Towards a better efficiency in N use, Bosch Eds.

North Atlantic millennial-scale climate variability 910 to 790 ka and the role of the equatorial insolation forcing.

On the Isotopic Chemistry of Carbonates and a Paleotemperature Scale.

Rapid Commun. Mass Spectrom.

Isotope ratio - Mass spectrometry

This article summarizes the configurations involving isotope ratio mass spectrometry (IRMS) technology available at the CCiTUB and the wide range of possible applications. Some examples of these applications are shown.Podeu consultar el llibre complet a: http://hdl.handle.net/2445/3216

RECERCAT

Isotope ratio - Mass spectrometry

Abstract

Similar works

Full text

Available Versions

Diposit Digital de la Universitat de Barcelona

RECERCAT