Pemanfaatan Ekstrak Ragi Dalam Kultur in Vitro Plantlet Media Anggrek

Abstract

. The aim of this experiment was to investigate the effect of yeast extract on the growth of dendrobium plantlets. Dendrobium is one of the favourite orchid genera. The orchid plantations generally use orchid seedlings that are obtained through in vitro culture. Media optimization is a critical point for improving the capacity of seedling mul- tiplication and quality. One of the methods to enrich the medium was the use of a complex organic mixture of yeast ex- tract. The materials used in this research were dendrobium orchid plantlets that were cultivated in Vacin & Went medium with the addition of 150 ml/l of coconut water, 20 g/l of sucrose and 50 g/l of banana pulp. The treatments were laid in a randomized block design with nine treatments and three replications. The treatments were the addition of yeast extract into the culture medium of concentrations of 0 (control); 0.25; 0.50; 0.75; 1.00; 1.25; 1.50; 1.75; and 2.00 g/l. The results showed that the use of yeast in the culture media significantly increased growth of dendrobium plantlets. Concentration of 1.25 g/l was the best for the performance of plantlets in term of plants height, leaf size, root length and number of roots