: Limbic system consists of several subsystems with their own roles to back-up human emotion. Human emotion can be observed through facial expression which is controlled by musculi facialis. One of the tools that are used to determine basic emotion of human through facial expression is Facial Action Coding System (FACS) and its action units (AUs). This study aimed to obtain musculi facialis that oftenly and rarely be used by Prabowo and his emotion duringthe first session of 2014-Presidential election debate. This was a retrospective descriptive study. Samples were 30 photos of Prabowo's emotional expression. The observation was performed by using FACS. The results showed that the most commonly used AU was AU 4 (26.92%), meanwhile the most rarely used AUs were AU 9 and AU 29, both were 0.96%. The obtained emotional expressions were happy (6.67%), sad (6.67%), fear (6.67%), angry (46.67%), surprised (3.33%), and disgusted (3.33%). Conclusion: The most commonly used musculus facialis was corrugator supercilii whereas the most rarely used ones were levator labii superioris alaquae nasi and masseter. The emotional expressions, consecutively from the most commonly to the most rarely observed, were angry; happy, as well as sad and fear, and surprised as well as disgust