Xylooligosaccharides (XOS) are prebiotic, functional food ingredients, with biological benefits
such as immunomodulatory and anti-inflammatory properties, anticancer and antioxidant activity.
Usually, XOS are produced from xylan by the combination of pre-treatment, enzymatic, chemical
and/or auto-hydrolytic methods. Fungal xylanases are the most suitable for XOS production. Xylan
rich agro-industrial wastes (corn cob is one of them) are used as a substrate for fungal xylanase
production and as start material for xylan extraction. In situ XOS production by fungal growth on
xylan rich medium represents an attractive and advantageous approach but insufficient described till
now. This method has many advantages over the others because bypasses the extraction and
purification of xylan and enzymes, it is environmentally friendly, low cost and time-consuming.
This study demonstrated A. tubingensis FAT35 has a great capacity for the synthesis of XOS using
corn cob as a substrate in solid state fermentation (SSF). Obtained XOS, during the fungal growth,
were characterized by TLC and HPLC. Significant antioxidant potential was shown by antioxidant
tests (ORAC, DPPH and FRAP). The obtained XOS are suitable to be a functional food additive
and are obtained in the simplest way that is both environmentally and economically suitable
