The objective of the present study was to identify the association of
the TNF-alpha- 308G/A and leptin receptor (LEPR) Gln223Arg polymorphisms
with the risk of development of type 2 diabetes mellitus (T2DM).
Methods: A total of 160 volunteers were studied: 108 with T2DM and 52
participants as control, who served as the control group. Polymerase
chain reaction-restriction fragment length polymorphism (PCR-RFLP) for
the genomic region of TNF-alpha- 308G/A and LEPR Gln223Arg were carried
out. Results: The frequency of LEPR Gln223Arg genotypes in T2DM and
control groups showed significant differences in the distribution of
genotypes (p < 0.05). The frequency also of TNF-alpha- 308G/A genotypes
in T2DM and control subjects showed significant differences in the
distribution of genotypes (p < 0.05). Conclusion: Our results indicate
that there are significant differences in the distribution of genotypes
and alleles between the individuals with T2DM and control subjects (p <
0.05)
