A reversed phase high performance liquid chromatographic method is
described that allows separation and estimation of the main neutral
lipid classes as well as several species of each class. By the
represented method waxes, hydrocarbons, fatty acids and their methyl
esters, sterols and their esters, free glycerylethers, fatty alcohols,
vitamin E and mono-, di- and triglycerides are separated into classes
and into class species within 55min.
A stepped gradient elution with methanol/water, acetonitrile/methanol,
acetonitrile/tetrahydrofuran and isopropanol/acetonitrile was performed
onto a reverse phase C18 HPLC column and the effluent was monitored by W
detection at 206 nm.
Application of the method in a plant extract and in a vegetable oil
sample is represented
