Md. Humayun, Kabir

東京農工大学Tokyo University of Agriculture and Technology博士（獣医学）Doctor of Philosophydoctoral thesi

Tokyo University of Agriculture and Technology Repository / 東京農工大学学術機関リポジトリ

S u m m a r y  o f  G r a d u a t i o n  /  D o c t o r a l  T h e s i s  A s s e s s m e n t  R e s u l t s   M d  H u m a y u n  K A B I R     To  co nt ro l  Ne w ca s t l e  d i sea se  (N D) ,  th e  a ppl i ca nt  in ve s t iga te d  va cc in at i o n  s ta t u s  a n d  ND o utb rea ks ,  e s tab l i sh e d  a  he at - re s i s ta n t  va cc in e  can d i dat e  s t ra i n ,  an d  e va lua te d  d i s i n fe c t i on  m at e r ia l s  t o  s t re ng th e n  b i o se cu r i t y.   In  Cha pt e r  I ,  he mag g l ut i na t i o n  (HA) -po s i t i v e  v i ru se s  f ro m th re e  ch i cke n  fa rm s  in  A fg ha ni s t an  w e re  i de nt i f i e d  as  N D v i ru s  (N D V)  b y  rev e rse  t ra n scr i p t i o n -po ly me ra se  cha i n  rea c t i o n  a ssay  a n d  sequ e n c i ng .  Th e  fu s i o n  (F )  pro te i n  g en e s  o f  t h e  t h ree  i s o la te s  sh ow n  in  t h e  phy lo ge n e t i c  an a ly s i s  i n d i ca te d  t hat  th e  i s o la t es  we re  h ig h ly  v i ru l e nt ,  b e l on ge d  t o  c la ss  I I  su b ge no ty pe  VI I  1 .1 .   In  Cha pt e r  2 ,  a  h ea t - re s i s ta nt  ND v a cc in e  can d ida t e  s t ra in  w as  s c ree n e d  a n d  ob t a in e d  Ve ro  ce l l  a da pte d  v i ru s ,  ND V-Ve ro20 .  T he  s t ra i n  i n du ce d  n eu t ra l i z i ng  a nt ib o die s  i n  i mm u niz e d  ch i cks .    In  Cha pt e r  3 ,  t o  s t re ng th e n  b i o se cu r i ty,  a  mi xt u re  o f  q ua te rna ry  amm o niu m co m pou n d (Q AC)  a n d  f oo d  a ddi t i v e  g ra de  ca l c iu m hy drox i de  (F dCa (O H) 2 )  w i t h  m et h an o l - co nt a i n in g  ant i f re e ze  wa s  exa mi ne d  a t  -20 ° C i n  su spen s i o n  t e s t s .  T he  m ixt u re  i na c t iva t e d  N D V wi t h i n  30  mi n  a t  -20 ° C.  T he re fo re ,  i t  i s  r e com me n de d  t ha t  QAC wi th  FdCa (O H) 2  b e  u se d ,  e spe c ia l l y  i n  win te r.    Th e  abo ve  do c t o ra l  d i s se r ta t i o n  i s  co n s i de re d  to  be  a  v e ry  s ig n i f i ca n t  con t r ibu t i o n  t o  th e  N D co n t ro l .   Al l  m em be rs  o f  t he  Boa rd  o f  t h e  Th e s i s  Asse ssme nt  Co mmi t t e e  o f  TU AT u na ni mo u s ly  a pprov e d  th i s  Do c to ra l  T h e s i s  (Do c to r  o f  Phi l o so ph y ) ,  f o r  i t s  nu m e ro u s  f i n d i ng s  cov e re d ,  co nt e nt s  an d  s t ru c tu re ,  a n d  t h e  nu mb e r  o f  pub l i she d  a r t i c l e s .  

ニューカッスルビョウウイルスノブンシテキシキベツトワクチンセッシュオヨビバイオセキュリティキョウカニヨルボウジョニカンスルケンキュウ

https://tuat.repo.nii.ac.jp/?action=repository_action_common_download&item_id=2072&item_no=1&attribute_id=16&file_no=3

ニューカッスルビョウウイルスノブンシテキシキベツトワクチンセッシュオヨビバイオセキュリティキョウカニヨルボウジョニカンスルケンキュウ

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