The data presented in this article are related to a previously published research article titled The timing of worm exclusion in dogs repeatedly infected with the cestode Echinococcus multilocularis (Kouguchi et al. 2016). This data describe a comparison of worm exclusion in the early stage of infection (1 day and 6 days post-infection) between dogs infected for the first time (control group) and dogs repeatedly infected with the parasite 4 times (repeated infection groups). We observed that 6 days post reinfection, the number of adult worms in repeated-infection groups decreased by 88.7% compared with the control group. Histological analysis comparison of the small intestinal mucosa from healthy, first infected, and repeatedly infected dogs are also reported. We observed no clear pathological abnormality, except the shortening of microvillus in reinfected dogs. However, eosinophil accumulation and eosinophilic ulcers were observed in some reinfected dogs. This data could be useful as preliminary data to develop a final host vaccine for this parasite

Furuoka, Hidefumi

Irie, Takao

Kouguchi, Hirokazu

Matsumoto, Jun

Morishima, Yasuyuki

Nakao, Ryo

Nonaka, Nariaki

Okubo, Kazuhiro

Yagi, Kinpei

English

DigitalCommons@University of Nebraska

University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln H. W. Manter Laboratory Library Materials Parasitology, Harold W. Manter Laboratory of 2020 Adult Worm Exclusion and Histological Data of Dogs Repeatedly Infected with the Cestode Echinococcus multilocularis Hirokazu Kouguchi Hokkaido Institute of Public Health Hidefumi Furuoka Obihiro University of Agriculture and Veterinary Medicine Takao Irie Hokkaido Institute of Public Health Jun Matsumoto Nihon University Ryo Nakao Hokkaido University See next page for additional authors Follow this and additional works at: https://digitalcommons.unl.edu/manterlibrary  Part of the Parasitology Commons Kouguchi, Hirokazu; Furuoka, Hidefumi; Irie, Takao; Matsumoto, Jun; Nakao, Ryo; Nonaka, Nariaki; Morishima, Yasuyuki; Okubo, Kazuhiro; and Yagi, Kinpei, "Adult Worm Exclusion and Histological Data of Dogs Repeatedly Infected with the Cestode Echinococcus multilocularis" (2020). H. W. Manter Laboratory Library Materials. 96. https://digitalcommons.unl.edu/manterlibrary/96 This Article is brought to you for free and open access by the Parasitology, Harold W. Manter Laboratory of at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in H. W. Manter Laboratory Library Materials by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Authors Hirokazu Kouguchi, Hidefumi Furuoka, Takao Irie, Jun Matsumoto, Ryo Nakao, Nariaki Nonaka, Yasuyuki Morishima, Kazuhiro Okubo, and Kinpei Yagi This article is available at DigitalCommons@University of Nebraska - Lincoln: https://digitalcommons.unl.edu/manterlibrary/96 Data ArticleAdult worm exclusion and histological data ofdogs repeatedly infected with the cestodeEchinococcus multilocularisHirokazu Kouguchi a, *, Hidefumi Furuoka b, Takao Irie a,Jun Matsumoto c, Ryo Nakao d, Nariaki Nonaka d,Yasuyuki Morishima e, Kazuhiro Okubo a, Kinpei Yagi aa Department of Infectious Diseases, Hokkaido Institute of Public Health, N19 W12, Kita-Ku, Sapporo,Hokkaido, 060-0819, Japanb Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine,Inada-cho, Obihiro, Hokkaido, 080-8555, Japanc Laboratory of Medical Zoology, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-0880, Japand Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine,Hokkaido University, Sapporo, Hokkaido, 001-0020, Japane Department of Parasitology, National Institute of Infectious Diseases, Tokyo, 162-8640, Japana r t i c l e i n f oArticle history:Received 15 January 2020Received in revised form 20 February 2020Accepted 24 February 2020Available online 2 March 2020Keywords:Echinococcus multilocularisVaccineRepeated infectionDogFinal hostMucosal immunityHistological analysisa b s t r a c tThe data presented in this article are related to a previously pub-lished research article titled “The timing of worm exclusion in dogsrepeatedly infected with the cestode Echinococcus multilocularis”(2016) [1]. This data describe a comparison of worm exclusion inthe early stage of infection (1 day and 6 days post-infection) be-tween dogs infected for the first time (control group) and dogsrepeatedly infected with the parasite 4 times (repeated infectiongroups). We observed that 6 days post reinfection, the number ofadult worms in repeated-infection groups decreased by 88.7%compared with the control group. Histological analysis comparisonof the small intestinal mucosa from healthy, first infected, andrepeatedly infected dogs are also reported. We observed no clearpathological abnormality, except the shortening of microvillus inreinfected dogs. However, eosinophil accumulation and eosino-philic ulcers were observed in some reinfected dogs. This data* Corresponding author.E-mail address: kouguchi@iph.pref.hokkaido.jp (H. Kouguchi).Contents lists available at ScienceDirectData in briefjournal homepage: www.elsevier .com/locate/dibhttps://doi.org/10.1016/j.dib.2020.1053532352-3409/© 2020 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).Data in brief 29 (2020) 105353could be useful as preliminary data to develop a final host vaccinefor this parasite.© 2020 The Author(s). Published by Elsevier Inc. This is an openaccess article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).1. Data descriptionThe data presented here were collected to elucidate the mechanisms underlying worm exclusion indogs repeatedly infected with E. multilocularis. The decrease in the number of adult worms inrepeatedly infected dogs is shown in Table 1. Dogs infected for the first time (control group) showed aworm burden ranging from 29,950 to 55,175. Six days post reinfection, the worm burden in dogsrepeatedly infected four times with E. multilocularis (dogs R1-R5) showed a burden ranging from0 (below detection limit) to 9450. Thus, the mean number of worms significantly decreased by 88.7% inthis group, compared to the control (p < 0.05). One day post-infection, the worm burden of dogsrepeatedly infected four times ranged from 2650 to 64,738 (dogs R6-R10). Compared to the control, themean number of worms decreased by 41.1%, although this decrease was not statistically significant,probably due to the large distribution of worm numbers among the dogs in this group.Specifications TableSubject Immunology and MicrobiologySpecific subject area ParasitologyType of data TableFigureHow data were acquired The number of worms in dogs infected with E. multilocularis was counted bystereomicroscope. The histological analysis was performed by optical microscopicobservation.Data format RawAnalyzedParameters for data collection Worm numbers were compared between the first-infection group (dogs infected with E.multilocularis for the first time) and repeated-infection group (dogs repeatedly infectedwith the parasite 4 times). A comparison of the histological profile was performedbetween healthy, first infection, and repeated infection groups.Description of data collection Fourteen dogs were divided into two groups as followed: first-infection group (dogsinfected with E. multilocularis for the first time; n ¼ 4) and repeated-infection group(dogs repeatedly infected with this parasite 4 times; n ¼ 10). Necropsy of these 10 dogsrepeatedly infected with the parasite was performed at 1 day and 6days post reinfection.Sample tissue was taken out from the central part of the small intestine.Data source location Hokkaido Institute of Public Health, Sapporo, Japan, 4304058.80400N; 14119059.76900E00Data accessibility With the articleRelated research article Kouguchi H, Irie T, Matsumoto J, Nakao R, Sugano Y, Oku Y, Yagi K, The timing of wormexclusion in dogs repeatedly infected with the cestode Echinococcus multilocularis, JHelminthol, 10.1017/S0022149X15001169Value of the Data The data of histopathology changes may facilitate understanding of worm exclusion in dogs infected with Echinococcus. The data on worm exclusion during the early re-infection stage may contribute to the development of a vaccine for finalhosts living at highly endemic areas where human echinococcosis is prevalent. This data could contribute to clarify the exclusion mechanisms of various canine pathogens.H. Kouguchi et al. / Data in brief 29 (2020) 1053532In our previous report [1], through observations of mucus feces or diarrhea, we have proposed thatadult worm exclusion occurs at an early stage in dogs repeatedly infected with E. multilocularis. Thedata presented here directly demonstrated that adult worm exclusion was almost complete by day 6postere-infection. Modulation of host responses is an important strategy whereby parasites ensure tosettle and persist in the host [2]. Therefore, exclusion of adult worms at an early stage of infection maybe crucial for the development of a final host vaccine.A typical histological image of the small intestinal mucosa of each group is shown in Fig.1. Fig.1A showsthe comparison of a healthy dog, a dog infected with this parasite for the first time, and a dog repeatedlyinfected with the parasite four times (6 days post-infection). The microvillus of the infected dogs wasshortened compared with the healthy dog. There was no clear histopathological abnormality in the firstinfected- and in the reinfected dogs. The histopathological findings in the small intestinal mucosa of dogsinfected for the first time are shown in Fig. 1B and C. Fig. 1B shows no eosinophil accumulation around theworms in dog C2 and dog C4, while eosinophil infiltration was observed in some dogs (such as dog C1).Fig. 1D shows eosinophil accumulation around the worms in dogs R1 and R3, belonging to the repeatedlyinfected group observed 6 days post-infection. Fig. 1E shows an eosinophilic ulcer observed in dog R4.2. Experimental design, materials, and methodsThis study was performed in strict accordance with the National Institutes of Health guide for thecare and use of laboratory animals, and the protocol for the experiments on the animals was approvedby the ethics committee of the Hokkaido Institute of Public Health (permit number: K25e2 and K29-4).All surgeries were performed after sodium pentobarbital anesthesia, and every effort was made tominimize animal suffering.Fourteen dogs were divided into 3 groups as followed: dogs infected with E. multilocularis for thefirst time (first infection group, n ¼ 4), dogs repeatedly infected with the parasite 4 times and nec-ropsied 6 days after the final infection (n ¼ 5) and dogs repeatedly infected with the parasite 4 timesand necropsied 1 day after the final infection (n ¼ 5).For the first-infection group, 100,000 protoscoleces were orally administered. In this group, theinfection continued for 6 days. In the two groups of dogs infected repeatedly, 500,000 protoscoleceswere administered orally in the first, second, and third infections. To terminate each infection, 100 mgof praziquantel (2 tablets of Droncit®, Bayer-Animal Health) was administered 30, 34, and/or 35 dayspost-infection (dpi), as summarized in Table 1. After deworming, dogs were re-infected byTable 1Infection regimes to show the number of worms of Echinococcus multilocularis. *Statistical significance (p < 0.05).Dog group Times of infection Worm number Age of dogs(at final infection)Period of infection(Days)Days betweeninfectionsControlC1 1 43,175 8 6C2 1 41,075 8 6C3 1 29,950 8 6C4 1 55,175 8 6Average 42,344*ReinfectionR1 4 9450 8 30, 34, 35, 6 14, 8, 14R2 4 N.D 8 30, 34, 35, 6 14, 8, 14R3 4 11,450 8 30, 34, 35, 6 14, 8, 14R4 4 3050 8 30, 34, 35, 6 14, 8, 14R5 4 50 8 35, 35, 35, 6 7, 7, 14Average 4800*ReinfectionR6 4 2738 8 35, 35, 35, 1 28, 7, 18R7 4 2650 8 35, 35, 35, 1 7, 7, 18R8 4 14,750 8 35, 35, 35, 1 9, 7, 20R9 4 64,738 8 35, 35, 35, 1 9, 7, 20R10 4 39,905 8 35, 35, 35, 1 7, 7, 14Average 24,956H. Kouguchi et al. / Data in brief 29 (2020) 105353 3administering protoscoleces at intervals ranging from 7 to 28 days (Table 1). For the fourth and finalinfection, 100,000 protoscoleces were orally administered. Following the final infection, the dogs wereeuthanized and the small intestine was removed, divided into six sections, and incubated in 100 ml ofDulbecco's modified Eagle's medium (DMEM) at 4 C for 7 days. Naturally released and scraped wormswere counted with a dilution of 1:125.3. MaterialsE. multilocularis (Nemuro strain) was obtained from a dog-cotton rat life cycle routinely maintainedat the Hokkaido Institute of Public Health (Sapporo, Japan). Protoscoleces were collected from cystsFig. 1. Histological analysis of E. multiocularis-infected dogs. Comparison of the small intestinal mucosa from healthy, first-infected,and fourth-infected dogs necropsied 6 days post-infection. Upper and lower panels show the HE and PAS staining, respectively (A).There are no clear responses in the small intestinal mucosa of first-infected dogs (B). In some dogs, eosinophil accumulation aroundinfected worms was found, as shown in panels (C) and (D). Eosinophilic ulcer observed in dog R4 is shown in (E). The arrows indicateeach eosinophilic response.H. Kouguchi et al. / Data in brief 29 (2020) 1053534developed in cotton rats 5e14 months after the infection. Beagle dogs and small intestine tissues fromhealthy dogs were purchased from KITAYAMA LABES CO., LTD. (Ina, Nagano, Japan).3.1. Histological analysisAll small intestine tissue used in this study were fixed in 10% formalin-PBS and embedded inparaffin wax. Cryosections were cut on a Retoratome REM-710 microtome (Yamato Kohki Co., Ltd.,Japan) at 25 C with a thickness of 4 mm and mounted on slides. The samples were further stained byHE and/or PAS staining and then washed with distilled water.Fig. 1. (continued).H. Kouguchi et al. / Data in brief 29 (2020) 105353 53.2. Data analysisData analysis was performed using the R software, version 2.15.2 (R Foundation for StatisticalComputing, Vienna, Austria). We performed a ShapiroeWilk normality test and observed that the datawere not normally distributed. Therefore, the non-parametric ManneWhitney U test was used tocompare the number of parasites between the groups. A p-value < 0.05 was considered statisticallysignificant.AcknowledgmentsThis work was supported by JSPS KAKENHI grants (numbers 17K08815) and MHLW Grants (H30-Shinkogyosei-Shitei-001). We thank K. Sotozaki, A Takeda and M Takeuchi (Sankyo Lab Service Cor-poration, Inc.) for their technical assistance.Conflict of InterestThe authors declare that they have no known competing financial interests or personal relation-ships that could have appeared to influence the work reported in this paper.References[1] H. Kouguchi, T. Irie, J. Matsumoto, R. Nakao, Y. Sugano, Y. Oku, K. Yagi, The timing of worm exclusion in dogs repeatedlyinfected with the cestode Echinococcus multilocularis, J. Helminthol. 90 (2016) 766e772, https://doi.org/10.1017/S0022149X15001169.[2] A. Rossi, J.M. Marques, C.M. Gavidia, A.E. Gonzalez, C. Carmona, H.H. García, J.A. Chabalgoity, Echinococcus granulosus:different cytokine profiles are induced by single versus multiple experimental infections in dogs, Exp. Parasitol. 130 (2012)110e115, https://doi.org/10.1016/j.exppara.2011.12.006.H. Kouguchi et al. / Data in brief 29 (2020) 1053536

Adult Worm Exclusion and Histological Data of Dogs Repeatedly Infected with the Cestode \u3ci\u3eEchinococcus multilocularis\u3c/i\u3e

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Adult Worm Exclusion and Histological Data of Dogs Repeatedly Infected with the Cestode \u3ci\u3eEchinococcus multilocularis\u3c/i\u3e

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