The data set presented in this article is related to a previous research article entitled, The timing of worm exclusion in dogs repeatedly infected with the cestode Echinococcus multilocularis (Kouguchi et al., 2016). This article describes the genes \u3e2 fold up- or down-regulated in the first- and repeated-infection groups compared to the healthy controls group. The gene expression profiles were generated using the Agilent-021193 Canine (V2) Gene Expression Microarray (GPL15379). The raw and normalized microarray data have been deposited with the Gene Expression Omnibus (GEO) database under accession number GSE105098

Furuoka, Hidefumi

Irie, Takao

Ishiwata, Kenji

Kouguchi, Hirokazu

Matsumoto, Jun

Nakao, Ryo

Yagi, Kinpei

English

DigitalCommons@University of Nebraska

University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln H. W. Manter Laboratory Library Materials Parasitology, Harold W. Manter Laboratory of 2018 Gene Expression Profiles of the Small Intestinal Mucosa of Dogs Repeatedly Infected with the Cestode Echinococcus multilocularis Hirokazu Kouguchi Hokkaido Institute of Public Health Takao Irie Hokkaido Institute of Public Health Jun Matsumoto Nihon University Hidefumi Furuoka Obihiro University of Agriculture and Veterinary Medicine Kenji Ishiwata Jikei University School of Medicine See next page for additional authors Follow this and additional works at: https://digitalcommons.unl.edu/manterlibrary  Part of the Parasitology Commons Kouguchi, Hirokazu; Irie, Takao; Matsumoto, Jun; Furuoka, Hidefumi; Ishiwata, Kenji; Nakao, Ryo; and Yagi, Kinpei, "Gene Expression Profiles of the Small Intestinal Mucosa of Dogs Repeatedly Infected with the Cestode Echinococcus multilocularis" (2018). H. W. Manter Laboratory Library Materials. 95. https://digitalcommons.unl.edu/manterlibrary/95 This Article is brought to you for free and open access by the Parasitology, Harold W. Manter Laboratory of at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in H. W. Manter Laboratory Library Materials by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Authors Hirokazu Kouguchi, Takao Irie, Jun Matsumoto, Hidefumi Furuoka, Kenji Ishiwata, Ryo Nakao, and Kinpei Yagi This article is available at DigitalCommons@University of Nebraska - Lincoln: https://digitalcommons.unl.edu/manterlibrary/95 Data ArticleGene expression profiles of the small intestinalmucosa of dogs repeatedly infected with thecestode Echinococcus multilocularisHirokazu Kouguchi a, Takao Irie a, Jun Matsumoto b,Hidefumi Furuoka c, Kenji Ishiwata d, Ryo Nakao e,Kinpei Yagi a,na Department of Infectious Diseases, Hokkaido Institute of Public Health, N19 W12, Kita-Ku, Sapporo,Hokkaido 060-0819, Japanb Laboratory of Medical Zoology, College of Bioresource Sciences, Nihon University, Fujisawa,Kanagawa 252-0880, Japanc Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine,Inada-cho, Obihiro, Hokkaido 080-8555, Japand Department of Tropical Medicine, The Jikei University School of Medicine, 3-25-8, Nishi-Shimbashi,Minato-ku, Tokyo 105-8461, Japane Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine,Hokkaido University, Sapporo, Hokkaido 001-0020, Japana r t i c l e i n f oArticle history:Received 22 November 2017Received in revised form19 December 2017Accepted 2 January 2018Available online 6 January 2018Keywords:E. multilocularisMicroarrayDogEchinococcosisVaccinea b s t r a c tThe data set presented in this article is related to a previousresearch article entitled “ The timing of worm exclusion in dogsrepeatedly infected with the cestode Echinococcus multilocularis”(Kouguchi et al., 2016) [1]. This article describes the genes 42-foldup- or down-regulated in the first- and repeated-infection groupscompared to the healthy controls group. The gene expressionprofiles were generated using the Agilent-021193 Canine (V2)Gene Expression Microarray (GPL15379). The raw and normalizedmicroarray data have been deposited with the Gene ExpressionOmnibus (GEO) database under accession number GSE105098.& 2018 The Authors. Published by Elsevier Inc. This is an openaccess article under the CC BY license(http://creativecommons.org/licenses/by/4.0/).Contents lists available at ScienceDirectjournal homepage: www.elsevier.com/locate/dibData in Briefhttps://doi.org/10.1016/j.dib.2018.01.0042352-3409/& 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license(http://creativecommons.org/licenses/by/4.0/).n Corresponding author.E-mail address: kinpei@iph.pref.hokkaido.jp (K. Yagi).Data in Brief 17 (2018) 180–183Specifications TableSubject area BiologyMore specificsubject areaGene expression studyType of data TableHow data wasacquiredAgilent-021193 Canine (V2) Gene Expression Microarray (GPL15379)Data format Raw, normalized, and analyzedExperimentalfactorsGene expression profiles were compared between healthy controls, first-infection,and repeated-infection groups.ExperimentalfeaturesThe relationship between the response at the small intestinal mucosa and wormexclusion in dogs repeatedly infected with E. multilocularis.Data sourcelocationHokkaido Institute of Public Health, Sapporo, Japan, 43 °04'58.804''N; 141 °19'59.769''E.Data accessibility The raw and normalized microarray data are available from the Gene ExpressionOmnibus (GEO) database under accession number GSE105098. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc¼GSE105098Value of the data The data present the differential gene expression profiles of the small intestinal mucosa of healthydogs and dogs infected with Echinococcus multilocularis. The data can be used to identify genes that might be involved in excluding the parasite from thesmall intestine of dogs. By comparing the first and repeated infection groups, the data could aid in the development of amucosal vaccine. The data could contribute to clarifying the exclusion mechanism(s) of various canine pathogens.1. DataThe present study was performed to elucidate the mechanisms underlying worm exclusion in dogsrepeatedly infected with E. multilocularis. The small intestinal mucosa gene expression profiles ofthree groups (healthy, first-infection, and repeated-infection) were compared. Total RNA was isolatedfrom the small intestinal mucosa, analyzed for quality and quantity, and used in microarray analysiswith the Agilent-021193 Canine (V2) Gene Expression Microarray (GPL15379; Agilent Technologies,Santa Clara, CA, USA). Compared to the control group, 1916 and 2950 genes were 42-fold up-regulated in the first-infection and repeated-infection groups, respectively, and 2418 and 3485 geneswere 42-fold down-regulated, respectively (Supplements 1 and 2).2. Experimental design, materials and methods2.1. Experimental designThis study was performed in strict accordance with the National Institutes of Health guide for thecare and use of Laboratory animals, and the ethics committee of the Hokkaido Institute of PublicHealth approved the protocol for the animal experiments (permit number: K25-2). All surgeries wereperformed under sodium pentobarbital anesthesia, and every effort was made to minimize suffering.Twelve dogs were divided into three groups (n¼4): the control group (healthy dogs), the first-infection group (dogs infected with E. multilocularis for the first time) and the repeated-infectionH. Kouguchi et al. / Data in Brief 17 (2018) 180–183 181group (dogs repeatedly infected with the parasite 4 times). The mucosal gene expression profilesbetween the three groups were compared by microarray analysis.2.2. MaterialsE. multilocularis (Nemuro strain) was obtained from a dog-cotton rat life cycle routinely maintained atthe Hokkaido Institute of Public Health (Sapporo, Japan). Protoscoleces (psc) were collected from cyststhat developed in cotton rats 5 –14 months following oral administration of 200 parasite eggs. The Beagledogs used in this study were purchased from KITAYAMA LABES CO., LTD. (Ina, Nagano, Japan).2.3. Sample preparationFor the control group, healthy dogs were euthanized and necropsies were performed. The smallintestine was removed and the central portion (approximately 15 cm) was washed with cold salineand then quickly treated with RNAlater Stabilization Solution (Thermo Fisher Scientific Inc., MA, USA)at 4 °C according to the manufacturer's instructions. These samples were preserved at –70 °C untiluse. For the first-infection group, 100,000 psc were orally administered to infect the dogs with E.multilocularis. In this group, infection continued for 6 days. For the repeated-infection group (n¼4),500,000 psc were administered orally for the first, second, and third infections. To terminate infec-tion, 100 mg of praziquantel (two tablets of Droncit, Bayer-Animal Health, Leverkusen, Germany) wasadministered 35 days post infection (dpi), as previously described [1]. Following deworming, the dogswere re-infected by administering psc at 8–14 day intervals (Table 1). For the final infection, 100,000psc were orally administered. After 6 days of infection, the central section of the small intestine ofdogs infected with E. multilocularis was removed and the tissue samples were treated using themethod described above.Tissue samples from dogs were carefully thawed in RNAlater at 25 °C. The small intestinal mucosawas scraped with a microspatula onto plastic culture dishes, and total RNA was extracted from thetissue samples using an RNeasy Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manu-facturer's instructions. In each group, equal amounts of purified total RNA from the four dogs werepooled. The quantity and quality of total RNA samples were determined using a Nanodrop 1000spectrophotometer. All samples had OD260/280 ratios of 1.97–2.05 with concentrations of 187.7–258.69 ng/µL. Agilent Bioanalyzer analysis demonstrated that all samples had RNA integrity numbersof 7.3–9.3 (Table 2). The samples were hybridized to the Agilent Canine (V2) array according to themanufacturer's instructions.Table 1Infection regimes for the three groups of dogs.Groups Total numberof infectionsNumberof dogsAge at finalinfectionInfection period(days)Days betweeninfectionsHealthy control 0 4 9First-infection 1 4 8 6Reinfection 4 4 8 30, 34, 35, 6 14, 8, 14Table 2Spectrophotometric data and RNA integrity numbers (RINs) of the RNA samples.Pooled sample OD260/280 OD260/230 Quantity (ng/µL) RINHealthy control 1.97 1.87 187.76 7.3First-infection 2.02 1.96 258.69 8.7Reinfection 2.05 1.75 249.25 9.3H. Kouguchi et al. / Data in Brief 17 (2018) 180–1831822.4. Microarray analysesThe microarray was conducted with a Canine (V2) array (Agilent) according to the manufacturer'sprotocol. Scanned images were analyzed with Feature Extraction Software 11.5.1.1 (Agilent; protocol:GE1_1105_Oct12 and Grid: 021193_D_F_20141110) using default parameters, to obtain backgroundsubtracted and spatially detrended processed signal intensities. Features flagged as feature non-uniform outliers were excluded. The microarray data were normalized using GeneSpring Ver.13.1.1software (Agilent; per chip: normalization to 75 percentile shift). Fold change (FC) analysis wasperformed using GeneSpring software with a threshold FC Z 2.0. The microarray gene list can befound on the Agilent Technologies web site (http://www.chem.agilent.com/cag/bsp/gene_lists.asp?arrayType¼gene).AcknowledgementsWe wish to thank DNA Chip Research Inc. for technical assistance, as well as K. Sotozaki, Y. Kasai,and A. Tamura (Sankyo Lab Service Corporation, Inc.) for their assistance.Funding sourcesThis work was supported by JSPS KAKENHI grants (Numbers 25460524 and 17K08815) and theResearch Program on Emerging and Re-emerging Infectious Diseases from Japan Agency for MedicalResearch and Development, AMED (17fk0108315h0003).Transparency document. Supporting informationTransparency data associated with this article can be found in the online version at https://doi.org/10.1016/j.dib.2018.01.004.Appendix A. Supporting informationSupplementary data associated with this article can be found in the online version at https://doi.org/10.1016/j.dib.2018.01.004.Reference[1] H. Kouguchi, T. Irie, J. Matsumoto, R. Nakao, Y. Sugano, Y. Oku, K. Yagi, The timing of worm exclusion in dogs repeatedlyinfected with the cestode Echinococcus multilocularis, J. Helminthol. 90 (2016) 766–772.H. Kouguchi et al. / Data in Brief 17 (2018) 180–183 183

Gene Expression Profiles of the Small Intestinal Mucosa of Dogs Repeatedly Infected with the Cestode \u3ci\u3eEchinococcus multilocularis\u3c/i\u3e

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Gene Expression Profiles of the Small Intestinal Mucosa of Dogs Repeatedly Infected with the Cestode \u3ci\u3eEchinococcus multilocularis\u3c/i\u3e

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