Portugal is one of the main producersof chestnut in Europe.info:eu-repo/semantics/publishedVersio

Afonso, Maria João A.P.S.

Dias, Rolando

Gomes, Catarina

Guerra, Nelson P.

Pereira, Ermelinda

Ramalhosa, Elsa

English

Biblioteca Digital do IPB

Effect of stirring and ultrasound-assisted extraction conditions in flavonoids, tannins, antioxidant and antimicrobial activities of chestnut outer shells (Castanea sativa Miller)IntroductionPortugal is one of the main producers ofchestnut (Castanea sativa Miller) in Europe.Chestnut shells are rich in total phenols andhydrolisable tannins. These compounds canprotect against the harmful effects of freeradicals, reducing the risk of several diseases ascancer, cardiovascular and neurodegenerativediseases, diabetes and inflammation.The demand for biologically active moleculeshas stimulated the search for naturalantioxidants.Several solvents have been employed in theextraction of chestnut by-products, such asmethanol, ethanol, acetone, or their aqueoussolutions, and water. Ethanol is considered a“GRAS” (Generally-Recognized-As-Safe) solvent,and the extracts obtained can be used in foodindustry.ObjectiveThis work aimed to study the role of theextraction conditions (solvent type, extractiontime and method) in the polyphenoliccompouds isolated from chestnut outer shells.The antimicrobial activity of the extracts werestudied using different types ofmicroorganisms.Afonso M.J.1; Dias R.2; Gomes C.P.2; Pereira E.L.2; Guerra N.P.3, Ramalhosa E.21Escola Superior de Tecnologia e Gestão, Instituto Politécnico de Bragança; 2Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Portugal3Facultade de Ciencias, Universidade de Vigo, Ourense, SpainMaterials and MethodologyInner shellOuter shell Groundchestnut outershell180 ºC, 1 hOvenFreezedryingORBITAL STIRRING EXTRACTION (SE)30 min and 60 minCastaneasativa MillerRoastedchestnutsRoasting10 g of chestnut outer shell+300 mL solventULTRASOUNDS EXTRACTION (US)1 min and 5 minRedissolved in the correspondingsolvent (10 mg/mL)- Ethanol:water, 80:20 (v/v)- Acetone:water, 80:20 (v/v)-WaterYIELD EXTRACTIONFLAVONOIDSHYDROLISABLE TANNINSTOTAL CONDENSED TANNINSANTIOXIDANT ACTIVITY-Total reducing capacity-DPPH-Reducing powerANTIMICROBIAL ACTIVITYRP-HPLCConclusionThe solvent and extraction method influenced the extractedcompounds. Good results were obtained with acetone:water and US.Acknowledgements: Foundation for Science and Technology (FCT, Portugal), FEDER and FEADER underProgram PT2020 [CIMO: UID/AGR/00690/2019] and [Go_ClimCast Project: PDR2020-1.0.1-FEADER-032060].Results and DiscussionAqueous acetone solution showed a better extraction yield andextracted significantly higher amounts of phenolics, flavonoids andcondensed tannins than the other solvents. Aqueous ethanol solutionrevealed to be a better solvent for the extraction of hydrolysabletannins.Extracts of ethanol:water and US methodshowed antimicrobial activity againstStaphylococcus aures, Bacillus cereus, Bacillussubtilis, Enterococcus faecalis and Candidaalbicans.Extracts obtained with acetone:water and USmethod showed a good antimicrobial activityagainst Proteus mirabilis.- Gallic acid- Ellagic acid- Tannic acids- …0100200300400500600Water Ethanol:water (80:20) Acetone:water (80:20)TPC (mg GAE/g extract)US_1 min US_5 min Stirring_30 min Stirring_60 mina, Aa, Aa, Aa, Aa, Ab, Bb, Ba, Ba, Ba, Ca, C a, C04080120160Water Ethanol:water (80:20) Acetone:water (80:20)CT (mg CT/g dry sample 180  C)US_1 min US_5 min Stirring_30 min Stirring_60 mina, Ab, Ba, Ab, Aa, Aa, b, Ab, Aa, b, Aa, Ab, c, Ba, Bc, B02004006008001000Water Ethanol:water (80:20) Acetone:water (80:20)HT (mg tannic acid/g extract)US_1 min US_5 min Stirring_30 min Stirring_60 mina, Ab, Ac, Ba, Ab, Aa, b, Bc, Cb, c, Ca, Ba, Ab, Bb, B0200400600800Water Ethanol:water (80:20) Acetone:water (80:20)Flavonoids (mg QE/g extract)US_1 min US_5 min Stirring_30 min Stirring_60 minb, Cb, Ac, Ab, Aa, Aa, Ac, B c, Bb, Bc, Ba, Ca, C

Effect of stirring and ultrasound assisted extraction conditions in flavonoids, tannins, antioxidant and antimicrobial activities of chestnut outer shells (Castanea sativa Miller)

https://bibliotecadigital.ipb.pt/bitstream/10198/26620/1/POSTER_ISEKI_.pdf

Effect of stirring and ultrasound assisted extraction conditions in flavonoids, tannins, antioxidant and antimicrobial activities of chestnut outer shells (Castanea sativa Miller)

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