This report presents the results of striped bass (Morone saxatilis) tagging and monitoring activities in Virginia during the period 1 September 2015 through 31 August 2016. It includes an assessment of the biological characteristics of striped bass taken from the 2016 spring spawning run, estimates of annual survival and fishing mortality based on annual spring tagging, and the results of the study that documents the prevalence of mycobacterial infections of striped bass in Chesapeake Bay. Also included is an investigation on the potential use of close-kin analyses to determine the size of the spawning stock in the Rappahannock River. The information contained in this report is required by the Atlantic States Marine Fisheries Commission and is used to implement a coordinated management plan for striped bass in Virginia, and along the eastern seaboard