Microbial degradation of complex aromatic mixtures and analysis of biodegradation products using comprehensive two-dimensional gas chromatography-mass spectrometry

Abstract

Ugljovodonici nafte i poljoprivredni lignocelulozni otpad neke su od najčešćih zagađujućih supstanci u životnoj sredini, a zajednički im je složen hemijski sastav i prisustvo velikog broja aromatičnih jedinjenja koja ispoljavaju toksične efekte. Cilj ove disertacije bio je proučavanje razgradnje složenih smeša aromatičnih supstanci i to aromatične frakcija dizel goriva D2 (AFD) i komine grožđa koja nastaje u procesu proizvodnje vina. Za ispitivanje biodegradacije korišćena je sveobuhvatna dvodimenzionalna gasna hromatografija sa masenom spektrometrijom (GCxGC-MS). Za razliku od konvencionalne gasne hromatografije, ovom tehnikom se sa većom rezolucijom razdvajaju analiti, pa je pogodnija za analizu složenih smeša...Petroleum hydrocarbons and agricultural lignocellulosic waste are some of the most common environmental pollutants. They have a very complex structure and are composed of a large number of different aromatic compounds that have toxic effects. The aim of this dissertation was to study the degradation of complex aromatic mixtures, namely diesel fuel D2 aromatic fraction (AFD) and grape pomace obtained from the winemaking process. For the biodegradation analysis, comprehensive twodimensional gas chromatography-mass spectrometry was used (GCxGC-MS). Compared with conventional gas chromatography, this technique has a greater separation resolution, thus, it is more successful in the analysis of complex mixtures..