Lugt, Charles Bruno

English

The mutual synergistic activity of digitoxin and verdoxin has in recent years led to an interest in Digitalis pururea L. races with a high content of the verdoxin complex. In order to select such races, answers were required to the following quistions: Is the total verdoxin complex (glucoverodoxin + verodoxin) genetically determined, as it is in the case with other glycosides in the plant, an/or does it depend on individual development of the plant? ... Zie: Summar

ARTS repository - University of Groningen

   University of GroningenVariability in the occurence of formylated diditalose glycosides in Digitalis purpurea L.Lugt, Charles BrunoIMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite fromit. Please check the document version below.Document VersionPublisher's PDF, also known as Version of recordPublication date:1975Link to publication in University of Groningen/UMCG research databaseCitation for published version (APA):Lugt, C. B. (1975). Variability in the occurence of formylated diditalose glycosides in Digitalis purpurea L.s.n.CopyrightOther than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of theauthor(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).The publication may also be distributed here under the terms of Article 25fa of the Dutch Copyright Act, indicated by the “Taverne” license.More information can be found on the University of Groningen website: https://www.rug.nl/library/open-access/self-archiving-pure/taverne-amendment.Take-down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediatelyand investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons thenumber of authors shown on this cover page is limited to 10 maximum.Download date: 13-02-2023SummarvINTRODUCTION and SCOPEThe mutual slrrergist j .c act ivi ty of digitoxin and verodoxin has in recent years1ed to an interest in Digital is purpurea L. races with a high content of theverodox-n corplex. In order to select such races, answers were required to thefol lorving questions:Is the total verodoxin conrple.x (glucoverodoxin + verodoxin) genetically deter-m i n c d ,  a s  i t  i s  t h e  c a s e  w i t h  o t h e r  g l y c o s i d e s  i n  t h e  p 1 a n t ,  a n d / o r  d o e s  i tdepend on the individual development of the plant?In order to be able to answer these questions it was necessary to determinethe  co l ) ten t  o l ' the  pr imary  g lycos ide  g lucoverodox in  and the  secondary  g lyco-side verodoxin in the p1ant. A method was therefore developed for the quanti-tative determination of the most important cardj.ac glycosides in D. purpurea,among which are those of the verodoxin complex.CHAPTER 1. Car'riiac glycosi.des in Digitalis putpur'ea L,The rnost important cardiac glycosides occurring in D. purpullea are revierved.Thc methods which have been in use up ti11 now for the quantitative determi-n a t i o r r  o l  t h e s e  g l y c o s  i d e s  a r e  d  i s c u s s e d .CHAPTER 2. Materials and methodsThc :.rcthod worked out for the routine quantitative determination comprisesthe fol lowing steps: isolat ion of the above-mentioned glycosides from theplant material;  separation of the consti tuents of this glycoside mixture bymeans of thin-layer chronatography; visualizatton of the spots with fumingr ' " r -^ - r '1^ - ;^  ^^ : r '  ^ td  measurement  o f  the  f luorescence in tens i tv  o I  thc  snnfsr v r  r l  o ! l u ,  o r t u  l t q d J u l  q i l l s l l L  u l  ( l l g  I  l u u l  q ) L q t t l q  a l l L q t l )on stimulation with W 1ight.These fluorescence measurenents were carried out with a Zeiss TLC spectro-fluorirneter by comparison wi.th a reference mixture of the individual glyco-9 1sides to be measrrred as internal standard.On adding up the results of these measurements, they were found to be inagreement with those obtained by colorimetric determinations for the total. , . - . - . : r ^ ^  ^ .  ! L .  I  a l ] d  B  s e r i e s .t r / L u 5 a u u 5  u r  L r r c  n  iCIIAPTER 3. PLant matericls, cuLtiuatlon, and processingD. purpurea plants of dif ferent origins were investigated. The plants wereg lown in  i r  g reenhouse near  the  labora tory  o r  in  thc  gardcns  a t  Bu i tenpos tfFrieslandl .An experiment in which the leaves were dried at different tenlperatures showedthat the glycoside yield rvas highest iry-hen the leaves were dried at room tem-n p r a f r r r a  l ) r r r i n o  r f  a  O '  - ^ o5( ) "  and 70-  gave a  Lowcr  y ie ld .  The loss ,  however ,  wass m a l l ;  i t  w a s  s p r e a d  e v e n ) y  o v e r  a l l  t h e  g l y c o s i d e s  a n d  t h u s  d i d  n o t  a f f e c tthe results of the inr.rest igation. The effect of dif ferent condit lons ofstorage was also studied. I t  was found that the composit ion of the glycosidenixturc present in the rnaterial dried at 70o remainecl unchange<l.CHAPTER 4, App,L'[g;,7t.ort of the results of the prelzminary er1:erinentsSect ion  4 .1 .  D is t r ibu t ion  o f  the  g lycos ' tdes  in  the  mature  rose t teThe distr ibution of the dif felent glycosides in the mature rosette was in-vestigated, part ly in order to determine which part of the plalrt  could betakcn as a representative sanple in the later select ion work, The results ob-tained led to the fol lowing conclusions:- Although the total glycoside content is hi.ghest in the young leaves, thishas l i t t le effect on the amount of glycosides in the whole rosette which istictenlincd chiefly by the largcr rniddle leavcs.- Oldcr leaves contain relat ively more of B- and E-series glycosides than ofA - s e r i e s  g l y c o s  i d e s .- Proport ionately, the largest amount of digitalose glycosides occurs in theyourg leaves.- Relat ively, fonnylat ion takes place to the greatest extent i-n the older9 Z- The presence of the fonnylated digitalose glycoside verodoxin is determinedh '  r h a  ; , r f t ' ' ^ n - , ,  ̂ f  t h C S e  l a S t  t W O  f a c t n r s .  w h i c h  a c t  i n  o n n o s i t e  d i r e c -' , . /  . , . .  t  v v r r r L r rt ions. I t  is for r.his reason that relat ively the sreatest anount of vero-doxin is nevertheless found in the rniddLe leaves.- The first-mentioned conclusion indlcates that the middle leaves are thenost important in searching for chemical races with a high verodoxincontent.-  J u v e n i l e  f o r - m s  o f  t h e  p L r n t ,  a r t - i f  i c i a l  l y  m a i n t a i n e d  b y  c L o s e  p l a n t i n g ,cou ld  p rov ic le  a  g rea ter  v ie ld  o f  d ig i ta lose  g lycos ides .Section 4.2. Production of glycosides at cl i f ferent stages in the deuelopmenLof the plant, and the effect of certain planting techniquesApart frorn the effcct of the cult ivat ion methods, i t  was i"ntended here todctenninr: the most favourable moment for ensuring maxintul yleld of cardiacglycosides, From the resuLts the fol lolving conclusions were drawn:-  P r o v i d e d  c o n d i t i o n s  a r c  f a v o u r a b l e ,  s o w i n g  f r o m  s e e d  g i v e s  r i s c  t o  agreater yield of leaf material,  and of glycosides, per writ  area than doessett ing out young plants.- lhe arnount of A-series glycosides, explessed as weight per Lrnit  area, in-c reases  dur ing  growth  n lo re  than does  tha t  o [  the  o ther  g lycos ides ;  however ,t h e  r c l l r t i v e  a m o L r n t s ,  e x p r e s s e d  a s  p e r c e n t a g e s ,  i n d i c a t e  a  d i m i n u t i o n  i nt h c  A - s c r i e s  p l v c o s i d c s .- Nlature leaves have a relat ively high percentage of gitaloxin. This j .s to bes e e n  p a l t i c u J a r l y  i n  t h e  h i g h  c o n t e n t  i n  t l r o s e  p l a n t s  r v h i c h  h a v c  b e e n  p i a r r -ted out and the low content in those plants which have been raised fronseed, In the rosette of plants which have been planted out, these matureleaves predorninate,-  D u r i n g  g l o r v t h  o f  t h e  r o s e t t e ,  c o m p a r e d  w i t h  t h e  d i g i t o x o s e  g l y c o s i d c s ,  t h e. i  r g i t a l o s e  g l 1 ' c o s i . l e s  s h o r v  t l r e  s m a l  l c s t  i n c r e a s c .- The percentage of C-16 hydroxy glycosides increases during growth of theroset te .Section 4.3. The gLycosides dzLring the groatl t  of plants frotn dif ferent origzrtsAs a start to the selection procedure, a comparison was made betwccn D. pur-93purea pltrts of different origins. For the sampl.ing, the findings describedin section 4.1 were applied and mature leaves were taken for the purpose. Theinvestigation resulted in the fol lowing conclusions:- 0f the populations studied, the leaf yield of two of them, Krakow and Bom,was least affected by the external condit ions.- There are populations with high and populations with low contents ofcertain glycosides.- For each population there is a relative increase i.n the content of glyco-sides duri.ng growth. This increase ls due largely to an increase i-n theB-ser ies  g lycos ides .- The populations Bonn and wageningen were called A tlpes and those frornKrakow and Surrey B t1pes. The A t)?es are characterized by a percentageof A-series glycosides which is greater than that of the B- or E-seriesg lycos ides .- Although the different populations have different 1eve1s, during growth thetotal amount of E-series glycosides in a populat ion is maintained at a moreor less constant 1eve1.- with only slight variatlons between the different populations, the percen-tages of the B- aad E-series glycosides were found to be in the rat io j  :  1.- The populations tr{ageningen, Krakow, and surrey contain more di-gitaloseglycosides at the beglnning of the season than do the other populat ions.- At the beginning of the season, for the population Krakow the ratio ofbound digitalose to bound digitoxose is in favour of dlgitalose. For theother populat lons, this rat io is equal or against digitalose.- At the end of the season, the rat io of digitalose to digitoxose c-16 hy-droxy glycosides tend-s to the sane value for al l  the populat i .ons.- A cross between the populations from Krakow and fron lVageningen or surreycould lead to very good results.Fina11y, with regard to the questions posed in the introcluct j .on, the fol-lowing points may bc made:There are distinct differences between the 1eve1s of verodoxin accunulationin the seven different populations. It is also clear that the total anor.ntof glycosides belonging to the verodoxin complex is partly deterrnlned by theindividual development of the p1ant.9 4

Variability in the occurence of formylated diditalose glycosides in <i>Digitalis purpurea</i> L.

https://pure.rug.nl/ws/files/3453097/Lugt.PDF

Variability in the occurence of formylated diditalose glycosides in <i>Digitalis purpurea</i> L.

Abstract

Similar works

Full text

Available Versions

ARTS repository - University of Groningen