Boer, Huug

Caine, WR

Steendam, CA

Tamminga, S

van 't End, M

Verstappen, B

Verstegen, MWA

Visser, GH

The N-15-isotope dilution technique (N-15-IDT), with either pulse-dose oral administration or continuous i.v. administration of [N-15]-L-leucine (carotid artery), both at 5 mg/(kg body weight . d), was used to measure ileal (postvalve T-cecum cannula) endogenous nitrogen recovery (ENR) in pigs (9 +/- 0.6 kg). Diets were cornstarch, enzyme-hydrolyzed casein with no (control) or high (4%) content of quebracho extract (Schinopsis spp.) rich in condensed tannins. Blood was sampled from a catheter in the external jugular vein. Mean plasma N-15-enrichment at d 8-10 was higher (P = 0.0009) after i.v. than after oral administration [0.0356 vs. 0.0379 atom% excess (APE)]. Plasma 15N-enrichment for i.v. infused pigs was 0.01117 APE higher (P &lt;0.0001) and for orally dosed pigs 0.0081 APE lower (P &lt;0.0001) at 11 h postprandial compared with 1 h postprandial. Apparent ileal N digestibility was higher (P &lt;0.0001) for the control (85.5%) than for the quebracho diet (69.5%). ENR was calculated from the ratio of N-15-enrichment of plasma and digesta. The ENR for the quebracho diet was similar to300% higher than for the control diet (6.03 vs. 1.94 g/kg dry matter intake, P &lt;0.001). The real N digestibility (92.2 +/- 0.4%) was equal for both diets (P = 0.1030) and both tracer methods (P = 0.9730). We concluded that oral administration of [N-15]leucine provides reasonable estimates of ENR in pigs fed semipurified diets with high or low content of tannins; however, one must be careful in extrapolating this conclusion to studies with other protein sources or feeding frequencies.</p

ARTS repository - University of Groningen

Route of tracer administration does not affect real endogenous nitrogen recovery measured with the N-15-isotope dilution technique in pigs fed rapidly digestible diets

Route of tracer administration does not affect real endogenous nitrogen recovery measured with the N-15-isotope dilution technique in pigs fed rapidly digestible diets

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ARTS repository - University of Groningen