Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar
Abstract
This research aims to determine the highest protein content and the optimum activity of the
enzyme bromelain extracted from pineapple stem. This research was conducted at the Laboratory of
Microbiology Makassar Alauddin State Islamic University in May 2013. Variable in this study was
the isolation and measurement of bromelain enzyme activity as the independent variable while the
crude extract of pineapple stem by variations in pH as the dependent variable. The type of this
research was experiments with bradford method for determination of protein content enzyme
bromelain, with ammonium sulfate concentration variation for precipitation was 10-60%, as well as
the determination of the enzyme bromelain activity at variation pH was 4.0; 5.0, 6.0; 7.0 and 8.0, at
650C temperature with incubation time was 10 minutes. Each stage has done three times in repetition
and analyzed spectrometry. The results showed the highest levels of protein precipitation with
ammonium sulfate at 60% was 37,785 mg/ml. While the optimum pH of the enzyme bromelain
activity at pH 6.0 was 1.021 activity units /gra