In vitro culture of bell pepper [Capsicum annuum cv. Bryza] and its greenhouse performance

Abstract

Two methods of pepper regeneration regarding the rate of obtaining microcuttings were compared. In the first one, halves of imbibed seeds were cultured on Murashige and Skoog (MS) medium without growth regulators (GR). In the second one, seedling explants, cotyledon segments and shoot tips, which were cultured on MS medium with GR, were used. The induction of adventitious buds occurred on the cut surface of the explant and depended upon the type of the explants, the presence and concentration of GR. The microcuttings which were fit for the ex vitro use, developed mainly from adventitious buds formed on the explants derived from the seeds. Adventitious buds on the explants derived from the seedlings developed mainly into leaflike structures while the axillary buds - into shoots. Rooted shoots were grown successfully and potted out in a greenhouse where they grew into normal fruit bearing plants