Duarte, Rafael D. C.

Muller, Onno

Quirós-Vargas, Juan

Silva, Marta Nunes da

Vasconcelos, Marta W.

English

Repositório Institucional da Universidade Católica Portuguesa

Common bean (Phaseolus vulgaris L.) nutritional profile & soil microbiome community is affected by elevated atmospheric carbon dioxide (CO2)Rafael D.C. Duartea, Marta Nunes da Silvaa, Juan Quirós-Vargasb, Onno Mullerb, Marta W. Vasconcelosa*a Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia,Rua Diogo Botelho 1327, 4169-005 Porto, Portugalb Institute of Biogeosciences, IBG2: Plant Sciences, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany* Correspoding author e-mail: mvasconcelos@ucp.ptResults & DiscussionConclusionsAcknowledgementsIntroduction MethodsFACEFree Air CO2Enrichment University of BonnCampus Klein AltendorfGermanyLegume crops, such as common bean (Phaseolus vulgaris L.), are keycontributors of essential nutrients for human health while playing a pivotalrole in the sustainability of agroecosystems. Unfortunately, they are alsosensitive to rampant climate change nefarious effects, such as elevatedconcentrations of atmospheric CO2 (eCO2), exacerbating dietary challengesand the unsustainable use of agronomic resources.Thus, the need to unravel the intraspecific effects of eCO2 onbiomass yield, nutritional value, microbial communities and geneticregulation of food crops is of upmost importance to anticipate potentialshortcomings to human nutrition and expedite mitigation strategies.Phaseolus vulgaris L. genotypes ‘Logan’, ‘G1378’ and ‘Kazak’ ;Plant growth in field conditions (Jülich FACE platform, Germany) underambient CO2 (400 ppm, control aCO2) or elevated CO2 (600 pm, eCO2);At plant maturity, evaluation of biomass yield, mineral profile, microbialcommunities and related gene expression.Representation of the plotting area. Atmospheric CO2 sensor.Figure 3 - Heatmap of relative fold of expression of genes involved in plant nutrition, stress response and microbiome symbiosis:FRO2, FER1, NRAMP6, NRAMP7, ZIP1, ZIP16, ERN1, RAM1, RAM2, TCR1, TCR2, HLH54 and HLH148 in shoots and roots of Phaseolusvulgaris genotypes Logan and G1378 grown under ambient or elevated CO2. In green: no alteration or downregulation of geneexpression; in gray/red: upregulation of gene expressionFigure 4 – Proteobacteria mean relative frequency of Logan genotypes exposed to ambient or elevated CO2 for 15 (T1) or 30 days (T2). Each value represents the average ± SEM and asterisks indicate significant differences at p<0.05.Figure 1 – Shoot (A) and full pods (B) biomass of Logan, G1378 and Kazakgrown under ambient or elevated atmospheric CO2. Each value represents theaverage± SEM and asterisks indicate significant differences at p<0.05.Figure 2. Leaf and pod mineral concentration (important micronutrients) of Logan’, ‘G1378’ and ‘Kazak’genotypes. Each value represents the mean ± SEM. Asterisks represent statistically differences at p < 0.05.Under eCO2, TCR1 and TCR2, whichregulate stress response, weredownregulated in Logan roots andupregulated in the roots of G1378- highersusceptibility of this genotype to eCO2.FRO2 was upregulated in the leaves ofLogan, agrees with the higher [Fe]observed.eCO2 plant effects seemed to beregulated by genes involved not only inplant nutrition but also plant response tostress.3. Gene expression2. Mineral Profile1. Plant biomassExposure to eCO2 resulted in a significantincrease of plant biomass in Logan and G1378(between 1.2- to 2.1-fold). Kazak appeared to beless impacted.This work was supported by the European Union’s Horizon 2020 Research and Innovation Programme EPPN2020 and by the German Ministry of Education and Research (DPPN: Grant-Number: 031A053A/B/C).4. Microbial communityIntraspecific variability in responding to eCO2 Important phylum to thebiogeochemical nutrient cyclingplaying a role in enhancing plantgrowth (as previously observed)Proteobacteria reportedZn and Fe accumulation in Logan was significantlyreduced by eCO2 in grains, whereas in Kazak it decreasedin shoots and grains. Contrarily, in G1378 [Fe] increasedin both shoots and grains.Again, different genotypes displayed contrasting behaviors,with Logan and G1378 appearing to be more resilient tonutritional losses caused by eCO2Elevated atmospheric CO2 impairs common bean productivity and nutritional quality in an intraspecific manner.Genotypes such as Logan may present higher resilience to this environmental stress with shifts in soil microbiome.The identification of resilient genotypes could lead to the implementation of improved crops as an adaptative strategy tomitigate nutritional shortcomings under predicted eCO2 conditions.

Common bean (Phaseolus vulgaris L.) nutritional profile & soil microbiome community is affected by elevated atmospheric carbon dioxide (CO2)

https://repositorio.ucp.pt/bitstream/10400.14/39982/1/Poster_RafaelDuarte_Ciencia22_final.pdf

Common bean (Phaseolus vulgaris L.) nutritional profile & soil microbiome community is affected by elevated atmospheric carbon dioxide (CO2)

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