Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Abstract
Research on biosynthesis of glutathione (GSH) by yeast Saccharomyces cerevisiae was undertaken with the aim of achieving high content of this compound intracellularly. The effect of GSH-amino-acids free and GSH-amino-acids enriched media added alone or in combination at different concentrations were examined. Furthermore, the combined effect of carbon and sulphur were also investigated. Cultivations were performed in shake-flasks at 28°C for 24-48 h in a synthetic medium with saccharose and ammonium sulphate as the sources of carbon and sulphur. The strain Saccharomyces cerevisiae cultured in GSH-amino-acids enriched media accumulated about 11-20 mg GSH/g d:ry biomass and the content of biomass was 6.5-12.5 g dry biomass/dm3 after 24 h of cultivation. Cysteine added alone to the medium was confirmed as the key amino-acid responsible for the increased biosynthesis of GSH in the yeast cell. In addition, increase in the sulphur content exerted a marked positive effect on its biosynthesis