Fasciolosis caused by Fasciola spp. is considered the most important helminth infection of ruminants in
tropical countries. Anthelmintic resistance has become a global concern. This study compared the efficacy of the
commonly used anthelmintics, determined the toxicity level and any indication of resistance. Thirty two water buffaloes
naturally-infected with Fasciola spp. were used to determine the efficacy of triclabendazole (TBZ), albendazole (ABZ),
and bromofenofos (BRO) using Fecal Egg Count Reduction Test (FECRT). To test the toxicity of the drugs given, serum
glutamic-pyruvic transaminase (SGPT) was evaluated before and within one week after treatment. One dose
administration of ABZ registered an efficacy of 79.17%, 73.33% for TBZ and 70.83% for BRO. Efficacy in two dosetreatment
group was 83.33% for both BRO and ABZ, and 90.00% for TBZ. Two dose-treatment was effective for TBZ
(90%), ineffective for BRO and ABZ. SGPT levels were not significantly different between pre-treatment and posttreatment
across all treatments. Giving one or two doses of anthelmintics, at one month interval, does not increase the
efficacy of the three drugs tested. The study also implies that anthelmintic resistance may have developed in the animals