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Assessment of dopaminergic neuron degeneration in a C. elegans model of Parkinson's disease
Authors
K. Palikaras SenGupta, T. Nilsen, H. Tavernarakis, N.
Publication date
1 January 2022
Publisher
Abstract
Transgenic Caenorhabditis elegans that expresses the full-length wild-type human α-synuclein in dopaminergic neurons provides a well-established Parkinson's disease (PD) nematode model. Here, we present a detailed protocol to monitor and dissect the molecular underpinnings of age-associated neurodegeneration using this PD nematode model. This protocol includes preparation of nematode growth media and bacterial food sources, as well as procedures for nematode growth, synchronization, and treatment. We then describe procedures to assess dopaminergic neuronal death in vivo using fluorescence imaging. For complete details on the use and execution of this protocol, please refer to SenGupta et al. (2021). © 2022 The Author(s
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Pergamos : Unified Institutional Repository / Digital Library Platform of the National and Kapodistrian University of Athens
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oai:lib.uoa.gr:uoadl:3219282
Last time updated on 10/02/2023