Blood samples from 2620 dogs living in 7 different areas were used for
the detection of antibodies against Ehrlichia canis and Borrelia
burgdorferi and Dirofilaria immitis antigen. Previously published
seropositivity data against Leishmania infantum from the same samples
were also used to identify multiple-pathogen seropositivity. The
associations between seropositivity against each pathogen and gender,
age, utility, hair length, and region of residence were evaluated by
multivariate logistic regression analyses. The prevalence of
seropositivity against E. canis, B. burgdorferi, and D. immitis was
12.25%, 2.23%, and 5.96%, respectively. Double- and triple-pathogen
seropositivity was detected in seven different combinations. Age and
region of residence were strongly associated with seropositivity against
all pathogens. The association between seropositivity and the area of
residence highlights the need for regular testing of dogs for
vector-borne pathogens in areas with similar conditions to define
control measures
