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Genomic structure and expression analysis of the RNase κ family ortholog gene in the insect Ceratitis capitata
Authors
T.N. Rampias Fragoulis, E.G. Sideris, D.C.
Publication date
1 January 2008
Publisher
Abstract
Cc RNase is the founding member of the recently identified RNase κ family, which is represented by a single ortholog in a wide range of animal taxonomic groups. Although the precise biological role of this protein is still unknown, it has been shown that the recombinant proteins isolated so far from the insect Ceratitis capitata and from human exhibit ribonucleolytic activity. In this work, we report the genomic organization and molecular evolution of the RNase κ gene from various animal species, as well as expression analysis of the ortholog gene in C. capitata. The high degree of amino acid sequence similarity, in combination with the fact that exon sizes and intronic positions are extremely conserved among RNase κ orthologs in 15 diverse genomes from sea anemone to human, imply a very significant biological function for this enzyme. In C. capitata, two forms of RNase κ mRNA (0.9 and 1.5 kb) with various lengths of 3′ UTR were identified as alternative products of a single gene, resulting from the use of different polyadenylation signals. Both transcripts are expressed in all insect tissues and developmental stages. Sequence analysis of the extended region of the longer transcript revealed the existence of three mRNA instability motifs (AUUUA) and five poly(U) tracts, whose functional importance in RNase κ mRNA decay remains to be explored. © 2008 The Authors
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Last time updated on 10/02/2023