In this study, the antifungal and antibacterial effect of 6 different  acrocarpous mosses were tested in vitro aganist 8 different  microorganisms. For the extraction, ethyl alcohol, methyl alcohol, acetone and chloroform were used as solvents. While the highest antimicrobial effect was seen in methyl alcohol extracts, extracts of chloroform showed the lowest level of antimicrobial effect. Grimmia anodon Bruch &amp; Schimp. which is one of the acrocarp mosses used in this study, showed the highest activity in termsof the number of microorganism affected. Tortella tortuosa (Hedw.) Limpr. only has effect on Candida albicans ATCC 16231 strain. All the results were compared with standard antibiotic discs, ketoconazole (50 ìg), ampicillin (10 ìg), eritromycin (15 ìg) and vancomycin (30 ìg).Key words: Moss, acrocarpous, antimicrobial effect

Elibo, B

Ezer, T

Kara, R

Çelik, GY

Çolak, E

English

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African Journal of Biotechnology Vol. 10(6), pp. 986-989, 7 February, 2011 Available online at http://www.academicjournals.org/AJB DOI: 10.5897/AJB10.1998 ISSN 1684–5315 © 2011 Academic Journals     Full Length Research Paper  Antifungal and antibacterial effects of some acrocarpic mosses  Büşra Elibol1, Tülay Ezer1*, Recep Kara1, Gökçen Yuvəlı Çelik2 and Emel Çolak1  1Department of Biology, Faculty of Science and Arts, Niğde University, 51100, Niğde, Turkey. 2Department of Pharmaceutical Technology, Faculty of Pharmacy, Erciyes University,  38039, Kayseri, Turkey.  Accepted 30 December, 2010  In this study, the antifungal and antibacterial effect of 6 different acrocarpous mosses were tested in vitro aganist 8 different microorganisms. For the extraction, ethyl alcohol, methyl alcohol, acetone and chloroform were used as solvents. While the highest antimicrobial effect was seen in methyl alcohol extracts, extracts of chloroform showed the lowest level of antimicrobial effect. Grimmia anodon Bruch & Schimp. which is one of the acrocarp mosses used in this study, showed the highest activity in terms of the number of microorganism affected. Tortella tortuosa (Hedw.) Limpr. only has effect on Candida albicans ATCC 16231 strain. All the results were compared with standard antibiotic discs, ketoconazole (50 µg), ampicillin (10 µg), eritromycin (15 µg) and vancomycin (30 µg).  Key words: Moss, acrocarpous, antimicrobial effect.   INTRODUCTION  Bryophytes are the oldest known land plants in the world (Zinsmeister and Mues, 1987). They consist of three separate divisions, the Marchantiophyta (liverworts), Anthocerotophyta (hornworts) and Bryophyta (mosses) (Glime, 2007; Goffinet and Shaw, 2009). Among them, Bryophyta is the largest division of Bryophytes (84% of families and ~98% of species). Based on the morpho-logical characters (branching patterns and location of sexual organs), the Bryophyta has been divided into two major groups as acrocarpous mosses and pleurocarpous mosses. The acrocarpous mosses are generally resistant to drought, by contrast, pleurocarpous mosses are more sensitive to drought (Schofield, 2001).  Many bryophytes exhibit antimicrobial effects against fungi and bacteria (Frahm and Kirchhoff, 2002; Ilhan et al., 2006; Sabovljevic et al., 2006; Basile at al., 1998a, b; 1999; Scher et al., 2004; Subhisha and Subramoniam, 2005; Bodade et al., 2008; Dülger et al., 2009). Almost all species of bryophytes are not damaged by insect larvae, fungi, bacteria, slugs, snails and mammals (Asakawa, 2001) because, biological compounds like oligosaccha-rides, polysaccharides, sugar alcohols, amino  acids, fatty     *Corresponding author. E-mail: tuezer@gmail.com. Tel: 0 (388) 225 24 19. Fax: 0 (388) 225 01 80. acids, aliphatic compounds, phenylquinone and aromatic and phenolic substances in bryophytes are protected aganist these organisms (Asakawa, 1981, 1984, 1990, 2000). Therefore, bryophytes have the potential for medical use. Traditional medical use of bryophytes in China started more than 400 years ago. For example, Polytrichum which is acrocarpous moss and Fissidens species were used as diuretic and hair growth stimulating drugs in China (Asakawa, 1990). Moreover, North Ame-rican Indians used Bryum, Mnium, Philonotis and Polytrichum juniperinum which are acrocarpous mosses to heal burns, bruises and wounds (Asakawa, 1990; Ilhan et al., 2006). In this study, we investigated the antifungal and anti-bacterial effect of 6 different acrocarpous mosses (Syntrichia ruralis (Hedw.) F. Weber and D. Mohr, Grimmia anodon Bruch and Schimp., Pleurochaete squorrosa (Brid.) Lindb., Bryum capillare Hedw., Tortella tortuosa (Hedw.) Limpr. and Orthotrichum rupestre Schleich. ex Schwägr.) which were tested in vitro aganist 8 different microorganisms (Bacillus subtilis RSKK 244, Staphylococcus aureus Koag(+), Bacillus cereus 863, Salmonella sp. 23.1, Escherichia coli ATCC 36218, Pseudomonas aeroginosa ATCC 27853 and Sac-charomyces cerevisiae TP(3-2), Candida albicans ATCC 16231). The aim of this study was to determine the anti-fungal   and   antibacterial   effects   of   some  acrocarpic  Elibol et al.        987    Table 1. Antimicrobial activity of Bryophyte extracts in different solvents.  Moss Solvent Inhibition zone (mm) B. subtilis RSKK 244 B. cereus 863 Salmonella  sp. 23.1 S. aureus  Koag (+) S. cerevisiae  TP (3-2) C. albicans ATCC 16231 P. aeruginosa ATCC 27853 E. coli ATCC 36218 S. ruralis EA - - 8 ±0.3 - 7 ±0.3 - - - MA - - 9 ±1.0 - 7 ±0.0 - - 7 ±0.1 A - - - - - - - - C - - - - - - - - G. anodon EA 7 ±0.3 - 8 ±1.1 8 ±0.2 - - 6 ±0.0 7 ±0.2 MA 7 ±0.2 - 8 ±0.0 8 ±0.8 8 ±0.5 - 6 ±0.1 7 ±0.0 A 6 ±0.0 7 ±0.3 7 ±0.7 7 ±0.3 8 ±0.5 - - - C 7 ±0.5 7 ±0.0 8 ±0.2 - 9 ±0.5 - - - B. capillare EA - 7 ±1.0 8 ±0.0 8 ±0.0 - - 6 ±0.1 - MA - 8 ±0.5 8 ±0.1 8 ±0.2 8 ±0.5 - 6 ±0.1 - A - - 7 ±0.1 7 ±0.2 8 ±0.5 - - - C - - 8 ±0.0 - 8 ±0.0 - - - P. squarrosa EA - 8 ±1.6 - - 7 ±0.0 - - 8 ±1.4 MA - 8 ±0.3 - - 7 ±0.0 - - 7 ±0.8 A - 7 ±0.5 - - 7 ±0.5 - - - C - 8 ±0.0 - - - - - - T. tortuosa EA - - - - - - - - MA - - - - - - - - A - - - - - 7 ±0.0 - - C - - - - - - - - O. rupestre  EA - - - 6 ±0.0 7 ±0.5 - - 7 ±0.5 MA - 7 ±1.0 - - 9 ±0.7 - - - A 9 ±0.0 - - 6 ±0.2 - - - - C 7 ±0.8 6 ±0.1 - - 8 ±0.5 - - - Ampicillin  21 23 19 18 - - 21 22 Eritromycin  28 29 30 31 - - 29 29 Vancomycin  15 16 16 15 - - 14 - Ketoconazole  - - - - 24 21 - -  Values are mean ± SD of 2 different experiments. EA: ethyl alcohol; MA: methyl alcohol; A: acetone; C: choloroform; -: not detected; SD: standard deviation. 988         Afr. J. Biotechnol.    mosses and to make a contribution to the pharmaceutical botany studies to be done in the future in Turkey.   MATERIALS AND METHODS  Plant material   Plant materials of this study were collected from the Melendiz Mountain, Okçular village, (Niğde), Turkey, at an altitude of 1095 m, 37°53'44.73'' N, 34°31'26.21'' E, in March 2010 and PozənƗı, Şeker Pınarı place (Adana), Turkey, at an altitude of 845 m, 37° 27' 08.20'' N, 340 52' 03.60'' E, in May 2010. The specimens were identified by Dr. Tülay Ezer. Specimens are deposited in the Herbarium of the Biology Department, Niğde University, Niğde, Turkey.   Preparation of the extracts  Samples of plant were treated with 0.8% Tween 80 aqueous solution to remove epiphytic hosts found on the plant surface. Then, the samples were washed in tap and distilled water, and dried on filter paper. The samples were extracted with different solvents (ethanol, methanol, acetone and chloroform) for 15 min in a liquefier blender until homogenized. The extracts were centrifuged at 400 rpm and the supernatant was dried at 45°C. The samples were dissolved in 100 mg of the dry residue in 10 ml sterile dimethyl sulfoxide (Basile et al., 1998a).   Determination of antimicrobial activity  The plant extracts were tested for antibacterial and antifungal activi-ties through the disc diffusion method, according to the National Committee for Clinical Laboratory Standards (NCCLS, 1997). Mueller-Hinton Agar (MHA) (LAB M) and Sabouraud Dextrose Agar (SDA), (LAB M) sterilized and cooled to 4 to 50°C, were distributed in sterilized Petri dishes. The filter paper discs (6 mm in diameter, Whatman No:1) were individually impregnated with 20 µl of the extract solutions (filtered with a pore size of 0.45 µm) and then placed onto the agar plates, which had previously been inoculated with tested microorganisms (100 µl). Plates were inoculated with bacteria incubated at 37°C for 24 h and 30°C for 48 h for the yeast strains. The diameter inhibition zones were measured in mm. All the tests were performed in duplicate. Studies performed in duplicate and the inhibition zones were compared with those of reference discs. Reference discs used for control are as follows: Keto-conazole (50 µg), ampicillin (10 µg), eritromycin (15 µg) and vancomycin (30 µg).   RESULTS AND DISCUSSION  In this study, the antimicrobial effects of six plants (S. ruralis (Hedw.) F. Weber & D. Mohr, G. anodon Bruch & Schimp., P. squorrosa (Brid.) Lindb., B. capillare Hedw., T. tortuosa (Hedw.) Limpr. and O. rupestre Schleich. ex Schwägr), having four different solvent extract (ethanol, methanol, acetone and chloroform), were compared with standard antibiotics used as positive controls. Antimicro-bial activity of plant extracts in different solvents on test microorganisms are given in Table 1.  The results indicated that ethanolic and methanolic extracts   of    S.   ruralis   had   inhibition   effect   against      Salmonella, E. coli and S. cerevisiae, while acetone and chloroform extracts were inactive against all test micro-organisms. The extract of B. capillare was active against only five strains (B. cereus, Salmonella, S. aureus, P. aeruginosa and S. cerevisiae). The extract of P. squarrosa showed moderate activity against some strains but, choloroform extract had prohibitive effect against only B. cereus strain.  The acetone extract of G. anodon was active  against only C. albicans strain. O. rupestre extracts were inactive against Salmonella, P. aeruginosa and C. albicans.  The antimicrobial test results revealed that G. anodon extracts had a potential activity against all micro-organisms, except C. albicans. While the highest anti-microbial effect was shown in  methanol extracts, extracts of chloroform showed the lowest level of antimicrobial effect. The results showed that S. cerevisiae, Salmonella and B. cereus were found to be more sensitive than the studied test strains (S. aureus, B. subtilis and E. coli). We know that conventional antibiotics are generally more active against the gram positive bacteria than gram negative bacteria. However, these bryophyte samples showed inhibition effect against both the gram positive and negative bacteria. Our results revealed that  the selected bryophytes might possess a novel antimicrobial agents. Also, some researchers reported special anti-microbial activities of different bryophyte samples against gram-negative bacteria (Basile et al., 1998a, b; Đlhan et al., 2006; Bodade et al., 2008).  On the other hand, our study showed that selected bryophyte samples in our study  have antifungal activity against two selected fungi. While S. cerevisiae was sensitive against the five plant extracts, this strain was resistance aganist T. tortuosa extracts. However, acetone extract of T. tortuosa was only active against the C. albicans. The results obtained are similar to some resear-chers report that extracts from mosses displayed anti-fungal activities (Castaldo-Cobianchi et al., 1988; Bodade et al., 2008). This study help in the discovery of new antibiotics that could serve as selective agents against infectious disea-ses.   ACKNOWLEDGEMENTS  We are indebted to the Research Fund of Niğde Univer-sity for financial support (Project No: FEB2010/21).   REFERENCES  Asakawa Y (1981). 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Antifungal and antibacterial effects of some acrocarpic mosses

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Antifungal and antibacterial effects of some acrocarpic mosses

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