Root and stem extracts of Girardinia diversifolia exhibited varying degrees of activity against Bacillus pumilus, Staphylococcus aureus, Escherichia coli, Aspergillus niger, Candida albicans and Saccharomyces cerevisiae. Three compounds namely β-sitosterol, 7-hydroxysitosterol and 3-hydroxystigmast-5-en-7-one, were isolated from the petroleum ether root extract. The present study gives scientific credence to the traditional use of Girardinia diversifolia in the management of microbial infections.Keywords: Girardinia diversifolia, phytochemical, antimicrobialEast and Central African Journal of Pharmaceutical Sciences Vol. 14 (2011) 89-9