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HER-2 DNA quantification of paraffin-embedded breast carcinomas with LightCycler real-time PCR in comparison to immunohistochemistry and chromogenic in situ hybridization
Authors
M. Ntoulia Kaklamanis, L. Valavanis, C. Kafousi, M. Stathopoulos, E. Arapantoni, P. Mavroudis, D. Georgoulias, V. Lianidou, E.S.
Publication date
1 January 2006
Publisher
Abstract
Objectives: To compare the detection of HER-2 status by real-time PCR, on paraffin-embedded breast carcinomas, in respect to immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH). Design and methods: Paraffin-embedded breast carcinomas collected from 85 patients diagnosed with early stage breast cancer were analyzed for HER-2 gene amplification by real-time PCR and CISH, as well as for HER-2 protein expression by IHC. Results: HER-2 gene amplification was observed in 19 (22.4%) of 85 breast cancer patients by real-time PCR and in 19 (22.4%) of 85 patients by CISH. Strong (3+) HER-2 protein over-expression was observed in 13 (15.3%) out of 85 patients. Moreover, there were 4 out of 85 (4.7%) patients that had moderate (2+) HER-2 protein over-expression, while 68 out of 85 (80%) patients had no HER-2 protein over-expression by IHC. There were strong concordance rates between real-time PCR and IHC (79/85, 92.9%, p < 0.0001) and real-time PCR and CISH (77/85, 90.6%, p < 0.0001). The concordance rate between the three methods was 90.6% (p < 0.0001). Conclusions: Our data show that the results obtained for amplification of HER-2 by real-time PCR on the LightCycler are comparable to those obtained by IHC and CISH. © 2006 The Canadian Society of Clinical Chemists
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Last time updated on 10/02/2023