Objectives: To determine the time-course changes of cell-free plasma DNA
(cfDNA) following heavy exercise.
Methods: cfDNA concentration, C-reactive protein levels (hs-CRP), uric
acid concentration (UA), creatine kinase activity (CK) were measured
before and post-exercise (immediately post, 0.5 h, 1 h, 2 h, 3 h, 4 h, 5
h, 6 h, 8 h, 10 h, 24 h).
Results: cfDNA increased (15-fold) 30-min post-exercise and normalized
thereafter. hs-CRP increased (56%, p<0.001) 1 h post-exercise, remained
elevated throughout recovery (52-142%, p<0.0001), and peaked (200%
rise, p<0.0001) at 24 h post-exercise. UA and CK increased (p<0.05),
immediately post-exercise, remained elevated throughout recovery
(p<0.0001), and peaked (p<0.0001) at 24 h of post-exercise recovery.
Conclusions: cfDNA sampling timing is crucial and a potential source of
error following aseptic inflammation. (C) 2010 The Canadian Society of
Clinical Chemists. Published by Elsevier Inc. All rights reserved