This paper assessed the impact of motivation on job performance and satisfaction of healthcare workers in Federal Medical Centre Jalingo, Taraba State-Nigeria. Data were collected from the primary source using a survey research design. A sample of 80 respondents was drawn and used to represent the entire population of the study. The research instrument was validated and tested using Alpha Cronbach with a reliability coefficient of 0.80. Data collected were analyzed using descriptive statistics of frequency, simple percentage, mean and standard deviation. The Results showed that good leadership, recognition, good work-life balance, good company culture and royalty from management, good wages, good working conditions, promotion, financial benefits, and job security were identified as the main factors that motivate the job performance of health workers. The result also showed that motivation promotes quality service delivery, motivation raises job satisfaction, fosters persistency in reaching a specific goal, yields better results, motivation promotes effectiveness and efficiency, and encourages workers' self-confidence toward goal attainment. Lack of good leadership, uncertainties, insufficient availability of resources and opportunities, the attitude of workers, fear of success, and fear of failure were found in this study as the main factors militating against health workers’ motivation in Federal Medical Centre Jalingo, Taraba State. It is therefore recommended that: The management of Federal Medical Centre Jalingo should pay attention to the improvement of employee empowerment, benefits package; work conditions & recognition in connection with their job performance practices; there is a need for performance standards to be measured by criteria directly related to the job and derived from a thorough job analysis