лабораторные животные, анестезия, двухкомпонентная анестезия, опыт, острый опы

Abou Assi Weam

Shaito Hanaa

Undetermined

Repository of Vitebsk State Academy of Veterinary Medicine

  186  ских ошибок обуславливаются невнимательным отношением, про-ведением обследования животного с несоблюдением правил и т.д. Мы не можем сказать, что ветеринарный врач сразу становит-ся опытным. Только наблюдая, переживая и изучая реальные явле-ния, он постепенно совершенствует свое мастерство. А при сочета-нии собственных практических данных и данных, почерпнутых в специальной литературе, опыт становится более весомым. Если врач любит свое дело и постоянно стремится к активному накопле-нию знаний и умений, высокая квалификация ему обеспечена.   УДК 615.035.1 ABOU ASSI WEAM, student (Lebanon), SHAITO HANAA, student (Lebanon) Scientific director Ivanov A.V., assistant  Vitebsk state order of peoples’ friendship medical university Vitebsk, Re-public of Belarus  EXPERIENCE OF TWO-COMPONENTS ANESTHESIA  ON LABORATORY ANIMALS DURING AN ACUTE EXPERIMENT  The experience of using small laboratory animals for scientific pur-poses goes back for more than 2,000 years. Starting from the 4th centu-ry BC, scientists have done many experiments on animals, discovered and proved many fundamental postulates of modern science concerning biology, anatomy, physics, medicine, etc. In one year during experi-ments, scientists use from 50 to 100 million laboratory animal. All this demonstrates the importance of using laboratory animals for scientific purposes. However, experiments on animals have done a significant change to the positive direction over the past centuries, which almost depends on the achievements of clinical pharmacology (in particular vet-erinary). The main requirements for anesthesia on animals are: sufficient for a full-fledged experimental intervention, "manageability" of anesthe-sia, economical efficiency, accessibility in the territory of Belarus Repub-lic, ease of use. Currently, many drugs have been developed and implemented that can be used for anesthesia to a laboratory animal. However, not all drugs are registered in Belarus and can be used legally in scientific re-searches. The search for the optimal scheme for the use of these drugs is still of some scientific interest. The purpose of this work is to study the compliance with these re-quirements of the scheme for anesthesia (Xilavit + thiopental sodium), which is used in the Department of Operational Surgery and Topographic anatomy of VSMU for surgical interventions on animals for educational Иностранные студенты - белорусской науке : материалы VI Международной научно-практической конференции иностранных студентов и магистрантов, Витебск, 20 апреля 2021 г. / Витебская государственная академия ветеринарной медицины ; ред. Н. И. Гавриченко [и др.]. - Витебск : ВГАВМ, 2021.  187  and scientific purposes. At the current moment, 2020-2021, both drugs (Xilavit & thiopental-sodium) were certified and approved to be used in veterinary practice at the territory of Belarus Republic. In the 2020-2021, 40 “Chinchilla” rabbits weighing 3.5-4.0 kg of both sexes aged 6 months till 1-year-old, were being operated during a year in the operating department of the operational surgery and topo-graphical anatomy of VSMU, with the aim of teaching students the main stages of surgery or during an acute scientific experiment. These labora-tory animals were anaesthetized under the following scheme: introducto-ry anesthesia - xylasine hydrochloride "Xilavit" - 0.2 ml/kg of animal body weight (4 mg per 1 kg of animal weight) - subcutaneously. Then within 5-10 minutes - the waiting period - which allows the animal to reduce anxi-ety and relax, to place itself on the operating table and catheterize the peripheral vein. After 10 minutes a solution of 2.5% of sodium thiopental is intravenously injected until reaching the necessary stage of anesthesia (unconsciousness, moderate muscle relaxation, moderate bradypnoe, analgesia). During the operation, gradual micro-bolus injections of small volumes (0.5 ml - 1 ml) of sodium thiopental is used as the depth of an-esthesia decreases. The anesthesia and its course proceeded without any disturbances for 39 animals. There is a moderate reciprocal potentiating effect of these drugs. The dose of xylazine hydrochloride was constant, according to the specified dosage, and rarely exceeded 16 mg of the drug per an-esthesia. While the consumption of sodium thiopental directly depended on the age of the animal, mass, and duration of surgery ranged from 15 ml to 25 ml per anesthesia, which corresponds to 1 bottle of dry sub-stance "sodium thiopental" sterile - 1.0 g. The usage of xylazine hydro-chloride for premedication significantly simplified the manipulation with the animal. However, one laboratory animal had a breath stop after the injection of thiopenthal, which was fatal, although the dose was not high-er than the estimated dose but apparently, there was an individual intol-erance in this case. Therefore, the search of a reasonable scheme of anesthesiological procedure when performing an experiment on an animal is difficult to consider like fully completed. The infinite growing range of pharmacolog-ical drugs opens new horizons for researchers. So the successful combi-nation of already well-known drugs can have some success. The scheme used in our experiments (Xilavit + thiopental sodium) fully meets the requirements of anesthesia for animals participating in the scientific experiment. Certification at the territory of Belarus Republic, controllabil-ity and ease of use are its advantages. However, the issues of individual intolerance of the scheme components are not completely clear and re-quires additional researches.  Иностранные студенты - белорусской науке : материалы VI Международной научно-практической конференции иностранных студентов и магистрантов, Витебск, 20 апреля 2021 г. / Витебская государственная академия ветеринарной медицины ; ред. Н. И. Гавриченко [и др.]. - Витебск : ВГАВМ, 2021.

Experience of two-components anesthesia on laboratory animals during an acute experiment

https://repo.vsavm.by/bitstream/123456789/14302/1/k-2021-9-2-186-187.pdf

Experience of two-components anesthesia on laboratory animals during an acute experiment

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