Brucellosis is a zoonotic disease with a significant economic and public health impact, which particularly affects humans and animal species in developing countries relying on livestock production. This study was conducted to provide asero-prevalence of bovine brucellosis in the central part of Cameroon. Sera from randomly collected blood samples were screened for Brucella antibodies using the rapid Rose Bengal Plate Test (RBPT) and the indirect Enzyme Linked Immuno-Sorbent Assay(i-ELISA). A questionnaire was administered to butchers to trace the origin of each animal sampled and know the age and sex of these animals. Statistical significance was determined by Chi-Square test using SPSS v 20 software. A total of 460 cattle (both male and female) were screened. RBPT detected Brucella antibodies in 67 (14.63%) With iELISA, 41 (9, 4%) samples tested positive for detecting Brucella LPS antibodies for confirmation. Data such as animal ageand their origin were not significant; however, the sexwas statistically significant. Animals from Ngaoundere were found to be more affected than animals from Bertoua. Therefore, the overall sero-prevalence obtained was 67 (14.63%) for RBPT and 41 (9, 4%) for i-ELISA.