Anthurium magnificum Linden is a very popular ornamental plant due to its beautiful foliage.In this species, traditional propagation methods are not practical for its large-scale production,and there are no studies in the scientific literature on itsin vitro culture. The objective ofthis work was to achieve the formation of callus inAnthurium magnificum Linden. For this,the effect of different concentrations of 2,4-D and two types of explant (leaf sections andpetiole sections) was evaluated. The results showed that the highest percentages ofcallus formation were obtained in the petiole sections with 100% in all treatments. Thefoliar sections showed lower percentages of callus formation in relation to the petiole. Thecallus formed from both explants were translucent, with a watery appearance and ayellowish-white color, characteristics that are evidence of non-regenerable callus. However,plants were regenerated from callus formed from petioles sections. It was shown that it ispossible to form callus in this species. In this sense, the highest percentage and growth ofcallus were reached when sections of petioles and 0.3 mg l-1 2,4-D in the culture mediumwere used. This research constitutes the first scientific report on thein vitro culture ofthis specie
