Separation and Purification and Antioxidant Activity of Polyphenols from Sargassum horneri

Abstract

In order to study the separation and purification process and antioxidant activity of Sargassum horneri polyph-enols, on the basis of ultrasonic assisted extraction of Sargassum horneri polyphenols, macroporous adsorption resin column chromatography was used to separate and purify Sargassum horneri polyphenol extract, and VC was used as the control to analyze its antioxidant activity in vitro. The results showed that the macroporous adsorption resin LX-158 exhibited the best adsorption and analysis conditions. The equilibrium time for static adsorption and analysis was 5 h. The following circumstances were ideal for dynamic adsorption and analysis: The flow rate of crude extract and eluent was 3 mL/min, the loading volume was 10 mL, the eluent was a 40% ethanol solution, and the eluent volume was 120 mL. The purity of Sargassum horneri polyphenols increased from 7.52% to 40.31% under these conditions. Antioxidant activity studies revealed that varied doses of Sargassum horneri polyphenols had noticeable scavenging effects on DPPH radical, ABTS radical, superoxide anion radical, hydroxyl radical and Fe3+ reducing power. With the increase of polyphenol concen-tration, its antioxidant capacity improved, and the IC50 was 6.60 μg/mL, 75.70 μg/mL, 2.22 mg/mL, 5.62 mg/mL, respec-tively. The purification process is feasible and stable, and it can be used as a process condition for the purification of polyphenols from Sargassum horneri