Background: The pathogen Staphylococcus aureus is able to persist on biotic andabiotic surfaces through biofilm formation. Enterocin AP-CECT7121 has shownhomogenous bactericidal activity in vitro against Staph. aureus of food and clinicalorigin. Objective: assess the eradication of biofilm-associated cells of multiresistant clinical Staph. aureus. Methods: 7 multi-resistant Staph. aureus(HSSA32-HSSA38) isolated from blood cultures of inpatients, from Hospital RamónSantamarina (Tandil City, Argentina) during the period January-November 2016were studied. All included patients had hip prosthesis infections. Purified APCECT7121 was used. MICs determinations were done by the micro-liquid dilutionmethod. For bactericidal activity against planktonic cells, AP-CECT7121 wasadded to staphylococcal cultures (108 CFU/mL), in a 96-well plate, to finalconcentrations of 1xMIC and 4xMIC, and incubated at 37°C. CFU counts wereperformed at 0, 4, 8, and 24 h. For assessing bactericidal activity against Staph.aureus biofilm cells, staphylococcal suspensions in Brain Heart Infusion broth-1%glucose were added to the wells and incubated for 24 h at 37°C. Formed biofilmswere incubated with the enterocin (1XMIC and 4XMIC) for 1 and 24 h. After, CFUcounts of the biofilms were done. Results: bactericidal activity of AP-CECT7121against planktonic cell was detected between 1- 4 h of incubation. A dosedependent effect of AP-CECT7121 against staphylococcal biofilms was observed.AP-CECT7121 (4XMIC) showed bactericidal activity against biofilm cells, butcomplete killing was not achieved. Conclusion: AP-CECT7121 showed a significantefficacy against staphylococcal biofilms and constitutes a candidate for futureapplications in the prevention and treatment of biofilm-associated infectionsFil: Delpech, Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Bistoletti, Mariana. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; ArgentinaFil: Lissarrague, Sabina. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; ArgentinaFil: Cherjovsky, Mariana. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; ArgentinaFil: Baldaccini, Maria Beatriz. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; ArgentinaFil: García Allende, Leonardo. Universidad Nacional del Centro de la Pcia.de Bs.as.. Facultad de Ciencias de la Salud. Departamento de Clinica.; Argentina7th Congress of European MicrobiologistsEspañaFederation of European Microbiological Societie
