The higher levels of essential elements in soil may be caused in optimum yields and crop quality. So it seems to be necessary to examine different levels of nutrients like Fe and Zn on plants and their productivity. To evaluate effects of iron and zinc application on yield and yield components of Pimpinella anisum an experiment was conducted at the research farm of Urmia University in 2009. Treatments, iron application (0, 0.2, 0.4 and 0.6%) and zinc (0, 0.2, 0.4 and 0.6%), were arranged as factorial based on randomized complete block design with 3 replications. Results showed the significant effect of interaction between iron and zinc on the number of seed in per plant,1000 seed weight, biomass yield, seed yield and harvest index (HI). The maximum value of the 1000 seed weight (2.22 g) was obtained from 0% of Fe and 0.2% of Zn, whereas the minimum value of the 1000 seed weight (1.92 g) belonged to 2% of iron and 0% of zinc. The highest number of seed per plant (762), maximum value of biomass yield (2652 kg/ha) and highest of seed yield (1372 kg/ha) were obtained from 0.6 and 0.4 percent of Fe and Zn and the lowest number of seed per plant (272), maximum value of biomass yield (716 kg/ha) and highest of seed yield (470 kg/ha) were obtained from 0 and 0.6 percent of Fe and Zn, respectively. The highest HI (66.18) was obtained at control treatment and the lowest one (46.67) at both 0.4 percent of Fe and Zn. The essential oil percent increase in average values of Fe and Zn spraying. But accumulation of Fe and Zn were the maximum in higher levels of spraying