In Vitro Expression of BDNF GDNF NGF NT3 and NT4/5 Genes in Selegiline Induced Bone Marrow Stromal Cells

Abstract

Objective: Two types of stem cells are found in the bone marrow: hematopoietic stemcells and marrow stromal cells (MSCs). Is it possible to induce the differentiation of bonemarrow stromal cells into neural cells in vitro and subsequently transplant them into thebrain? This might help repair neural lesions observed in some neurodegenerative disoders such as Parkinson’s disease (PD).Materials and Methods: In this study, cultured MSCs were incubated in serum free mediumcontaining 10-8 M selegiline for 24 hours and cells were cultured for another 48 hoursin α minimal essential medium (α-MEM) containing 20% fetal bovine serum (FBS).Then selegiline-treated cells were immunostained for neuronal markers such as NF-200and TH.Results: Cell counting results showed that Selegiline at doses of 10-6, 10-7 and 10-8 Mincreased the mean percent of viable cells. The most effective dose of Selegiline for diferentiationof bone marrow stromal cells (BMSCs) was 10-8 M. Molecular studies indcatedthat the expression of BDNF, GDNF, NGF, NT3, and NT4/5 genes were increased inSelegiline-treated cells compared to non-treated group.Conclusion: BMSCs can be directed to a neural fate in vitro and can be considered as acell source in neurological disorders for autograft therapy