The absolute scale of the neutrino mass plays a critical role in physics at every scale, from the particle to cosmological. Measurements of the tritium endpoint spectrum have provided the most precise direct limit on the neutrino mass scale. In this Letter, we present advances by Project 8 to the Cyclotron Radiation Emission Spectroscopy (CRES) technique culminating in the first frequency-based neutrino mass limit. With only a cm3-scale physical detection volume, a limit of mβ<180 eV is extracted from the background-free measurement of the continuous tritium beta spectrum. Using 83mKr calibration data, an improved resolution of 1.66±0.16 eV (FWHM) is measured, the detector response model is validated, and the efficiency is characterized over the multi-keV tritium analysis window. These measurements establish the potential of CRES for a high-sensitivity next-generation direct neutrino mass experiment featuring low background and high resolution