Although it is thought that drug abuse causes changes to thyroid function, the exact mechanism is still not well defined. Therefore, after a preliminary study, the aim of this supplementary study was to investigate the effect of narcotic addiction on thyroid function by measuring TBG. Materials and Methods: This research was conducted as a case - control study on 106 narcotic addicts and 106 healthy controls in Tehran. For measurement of TBG were used from serum samples of these individuals. Results: The TBG amount in case group was significantly increased compared with the control group (p<0.005). Discussion and Conclusion: The result of this supplementary study showed that the narcotic addiction can affect the thyroid function by increasing TBG. Indeed, this finding confirmed the main result of our preliminary study