Differential Gut Bacteria in Phosphine Resistant and Susceptible Population of Tribolium castaneum (Herbst) and their Biochemical and Molecular Characterization
Not AvailableGut bacteria community associated with insects are crucial to understand their function in the physiology
of the host. The hypothesis of the present study was that gut microbiota in phosphine resistant population
inhabits phosphine degrading bacteria. The study demonstrated the differential gut bacteria in phosphineresistant
and susceptible populations in Tribolium castaneum. Phosphine bioassay of different populations
of T. castaneum revealed that Jagatsinghpur (Odisha, India) population had the highest LC50 value (1.104
mg/l). Further, gut bacteria were isolated and characterized through biochemical and molecular techniques.
Among nine isolates of bacteria from resistant and susceptible populations, six isolates belonged to gram
positive bacteria and three belonged to gram-negative. The 16S rRNA gene sequences displayed 96 to
100 per cent homology to other 16S rRNA gene of strains within the National Centre for Biotechnological
information (NCBI), Genbank. Among different bacteria strains, two, Bacillus subtilis and Staphylococcus
saprophyticus were reported from resistant populations. Other species belonged to Staphylococcus sp.,
Enterobacter sp., Lysinibacillus fusiformis, Klebsiella pneumonia (all four from resistant populations)
and Achromobacter sp (from a susceptible population). Present study provides a basis for elucidating the
role of the gut bacteria in the phosphine resistance and design novel strategies for the management of T.
castaneumNot Availabl
