Not AvailableStandardization of Bacillus thuringiensis var. kurstaki containing polymer microparticles using a coacervation method.Not Availabl

Chandrika KSVP

Duraimurugan P

Navya M

KRISHI Publications and Data Repository

 Society for Biocontrol Advancement & ICAR-National Bureau of Agricultural Insect Resources P.B. No. 2491 , H.A. Farm Post, Hebbal, Bellary Road, Bengaluru-560024Sixth National Conference on Biological Control: Innovative Approaches for Green India    3 - 5 March 2021  ICAR-NBAIR, Bengaluru12345  154 | P a g e       Standardization of Bacillus thuringiensis var. kurstaki containing polymer microparticles using a coacervation method  K.S.V. Poorna Chandrika, P. Duraimurugan and Navya Matcha  ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad, Telangana, 500030, India. Corresponding author E-mail: ksvp.chandrika@icar.gov.in   Bacillus thuringiensis (Bt) is an endospore forming bacterium comprising of different strains which have the ability to produce different types of toxins thereby targeting a wide range of pests. The potential of Bacillus thuringiensis var. kurstaki as a foliar spray to control insect pests has received much popularity. However, high costs of production, their short shelf-life and less persistent nature are major constraints. In order to maximize its potential there is a need to increase its persistence under various environmental conditions. In the present study, a novel concept of bench scale microencapsulation was standardized to produce microparticles can increase environmental persistence overtime has been targeted. The Btk isolate Bt-127 encapsulated with polymer microparticles have been prepared using a coacervation method. The pectin and chitosan polyelectrolytes after dissolving in solvent were used for encapsulation of Bt using UV-protectants as stabilizers. Viability and percent entrapment of Bt in the carrier has been done at various levels of preparation and drying of microparticles. The efficiency of methodology and concentration of polyelectrolytes were observed. It has been found that, Bt was 85% entrapped in polyelectrolytes. The Bt entrapped microparticles and Bt-127 isolate powders were screened against 7 day old Spodoptera litura larvae @ 1g/l on castor (DCH-519) by spray bioassay method. The highest mortality against S. litura of 100% was recorded in Bt-127 entrapped microparticles at 48 hours after treatment with very low feeding whereas Bt-127 isolate recorded only 96.67%. Thus, Bt-127 entrapped microparticles were found more promising when compared to Bt-127 isolate alone.         6th NCBC - 2021   S5-PP-09   S5-PP-08 Chellappan, Mani 153 S5-PP-09 Chandrika, Poorna, K.S.V. 154 S5-PP-09 Duraimurugan, P. 154 S5-PP-09 Matcha, Navya 154 S5-PP-10 Vijayakumar, Rangasamy 155 S5-PP-10 Patil, Jagadeesh 155 S5-PP-10 Linga, V. 155 S5-PP-11 Sowmya, E. 156 S5-PP-11 Hosamani, Arunkumar 156 S5-PP-11 Ramanujam, B. 156 S5-PP-11 Ghante, Vijaykumar, N. 156       S6-OP-01 Shanmugam, P.S. 157 S6-OP-01 Sridharan, S. 157 S6-OP-01 Sangeetha, M. 157 S6-OP-01 Vennila, M.A. 157 S6-OP-02 Subbiah, Gayathri 158 S6-OP-02 Nisha, P.R. 158 S6-OP-03 Nalini, T. 159 S6-OP-03 Sneka, S. 159 S6-OP-04 Selvan, Senthamarai, P. 160 S6-OP-04 Subaharan, Kesavan 160 S6-OP-04 Das, Tania 160 S6-OP-04 Subramanya, T. 160 S6-OP-04 Patil, Deepak 160 S6-OP-04 Sreehari, U. 160 S6-OP-04 Samuel, Philip 160 S6-OP-04 Senthoorraja, R. 160 S6-OP-04 Sowmya, M. 160 S6-OP-04 Kumar, Vinay 160 S6-OP-04 Bakthavatsalam, N. 160 S6-OP-05 Shreenivasa, K.R. 161 S6-OP-05 Nataraj, O.R. 161 S6-OP-05 Desai, Nagappa 161 S6-OP-05 Shankara, M.H. 161 S6-OP-06 Sowmya, M. 162 S6-OP-06 Nayyar, Nishitha 162 S6-OP-06 Subaharan, Kesavan 162 S6-OP-06 Senthoorraja, R. 162 S6-OP-06 Selavan, Senthamarai, P. 162 S6-OP-06 Kumar, Vinay, T.M. 162 S6-OP-06 Mahalakshmi, M.L. 162 S6-OP-06 Raghavendra, A. 162 S6-OP-06 Mohan, M. 162 S6-OP-06 Bakthavatsalam, N. 162 

Standardization of Bacillus thuringiensis var. kurstaki containing polymer microparticles using a coacervation method

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