Alvestad, René

Burgerhout, Erik

Difford, Gareth Frank

Durland, Evan

Heia, Karsten

Lindberg, Stein-Kato

Mota, Vasco C.

Noble, Christopher

Striberny, Anja

Verstege, Gerhardus C.

English

NOFIMA Repository

Applications of hyperspectral imaging for documenting smoltification status and welfare in Atlantic salmonS.-K. Lindberg1, G. Difford1,2, E. Durland1, K. Heia1, R. Alvestad1, E. Burgerhout1, V. C. Mota1, A. Striberny1, G. C. Verstege3 and C. Noble11Nofima AS, Muninbakken 9-13, Breivika, 9019 Tromsø, Norway2Norwegian University of Life Sciences, Universitetstunet 3, 1433 Ås, Norway3Tromsø Aquaculture Research Station, Skarsfjordvegen 860, 9391 Kårvik, Norway234Spectral imaging56Feature Number of fish scanned with HSINumber of fish with manual reference Indicator typeAgreement with manual WITrial 1 Dorsal fin injuries725 290 OWI 0.54Trial 2 Plasma chloride 849 120 LABWI 0.73Trial 3 Eye injuries 300 300 OWI 0.55Trial 4 Lice count 1124 1124 OWI 0.65Summary of trialsTrial 1: Active fin damage (splitting and/or haemorrhaging)73218012 39Polychoric correlation10Cox (1974). Estimation of the Correlation between a Continuous and a Discrete Variable. 12311Manual scoring of two scorersSpearman’s correlation: 0.56Interrater agreement: 0.66Cohen’s kappa: 0.40Manual scores and camera outputPolychoric correlation: 0.54Trial 2: SmoltificationPicture source: http://www.lizzieharper.co.uk/news/article/155/Natural_History_Illustration_Salmon_life_cycle©2018 Lizzie Harper ©2018 Lizzie HarperParr SmoltDebes et al., 2020Khaw et al., 2021Odei et al., 2020Experimental setup• Spectral imaging followed by a 24 hour seawater challenge− 15.02.2021 (40 fish x 2 replicates) – week 3 (sampling 2)− 08.03.2021 (40 fish x 2 replicates) – week 6 (sampling 3)− 22.03.2021 (40 fish x 2 replicates) – week 8 (sampling 4)• Spectral image features → Plasma chloride ion levels after seawater challenge• 80 % training set, 20 % test set14Feature extraction15YOLO V4BackgroundremovalAverageResults16Test set R2 = 0.73Summary and future work• Proof of concept• Individual health screening, e.g.:− Smoltification assessment− Sea lice infection level assessment− Delousing injury assessment− Early warning for infectious diseases• Better quality of life -> happier fish• Reduced mortality -> happier farmers17

Applications of hyperspectral imaging for documenting smoltification status and welfare in Atlantic salmon

https://nofima.brage.unit.no/nofima-xmlui/bitstream/11250/3037860/1/Presentation%2bAquaculture%2bEurope%2b2022.pdf

Applications of hyperspectral imaging for documenting smoltification status and welfare in Atlantic salmon

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