Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences
Abstract
The spatial organization of an eukaryotic genome plays an important role in the control of nuclear gene expression. The new Hi-C method allows investigation of the three-dimensional architecture of whole genomes. It has not been applied to study of the spatial configuration of a germ cell genome hitherto. Here we describe a protocol for production and quality control of Hi-C libraries from fibroblasts and sperm cells. Our results demonstrate that the Hi-C method can be used for studying the spatial organization of the densely packed sperm genome