Premature infants assisted with breathing apparatus should be in calm condition so that there is synchronization between the baby's breath and the breathing apparatus manifested by changes in heart rate, respiration rate and oxygen saturation. One way to make babies calm during the use of breathing aids is the provision of lullaby music therapy. The purpose of this study was to find out the effect of lullaby music therapy on heart rate, respiration rate and oxygen saturation in premature infants with breathing apparatus. This study used quasi experiment design with pre-post test without control group to 22 preterm babies selected by non probability sampling technique via purposive approach with criteria of infant with breathing apparatus, gestational age 24-36 weeks, no ischemic hypoxic ensepalofati. Samples taken were heart rate measurement, respiration rate and oxygen saturation before lullaby music therapy was given and after lullaby was given for 3 days. Data analysis used was t test dependent. The statistical results showed the difference in heart rate, respiration rate and oxygen saturation on the first day before lullaby music therapy was administered compared to the third day after lullaby music therapy was administered with a pvalue value <0.05 for heart rate, pvalue <0.05 for respiration rate and p value <0.05 for oxygen saturation. The provision of lullaby music was proven to make premature babies at peace and could be provided in other baby care unit with breathing support as an effort to sustain peace for premature babies