Background: Decrease or increase in blood HDL cholesterol levels affect smokers and nonsmokers. Cigarettes have chemicals that can lead to several health problems such as nicotine. Nicotine resulted in the production of very low density lipoprotein(VLDL) in order to reduce redundant high density lipoprotein cholesterol levels.Method: This type of researchis anobservationalstudyusing across sectional design. Thisstudy populationisstudents ofthe Faculty ofMedicine, University ofSamRatulangi7th semesterof the school year2012/2013with apopulation of150people. Sampling technique inthis research is byusingpurposivesamplingtechniques, smokersand nonsmokersobtainedusing a questionnaire.Result: Determinedthatthere is astatisticallysignificant differencebetweenHDLcholesterol levelsin theblood ofmalesmokersand nonsmokersp=0.49.Cinclusion: There isstatistically significantdifferences inHDLcholesterol levelsin theblood ofmalesmokersand nonsmokers