Graphene oxide (GO) was synthesized by Hummers' method before being reduced by ascorbic acid and then modified with Fe3O4. The obtained material was characterised using infrared (IR) spectroscopy and X-ray diffraction (XRD) pattern showing the characteristic peaks of rGO and Fe3O4. The rGO/Fe3O4 materials were employed as an electrode modifier on glassy carbon electrode. It was shown that the modification of GCE with rGO/Fe3O4 significantly enhanced the electro-active surface area of the electrode (SGCE = 3,1 mm2; SrGO/Fe3O4/GCE = 12,2 mm2). The electrochemical behavior of Rhodamine B on the modified electrode was investigated, illustrating that the oxidation of Rhodamine B involved 2 electrons and 1 proton in acidic solution, and 2 electrons and 2 protons in neutral and slightly basic solution