Diversity of Seven Glutenin and Secalin Loci within Triticale Cultivars Grown in France

Abstract

Although the endosperm storage protein of hexaploid triticale have already been analysed, the allelic diversity of glutenins and secalins remains to be described. Analysis by SDS-PAGE of the majority of hexaploid triticales (69 cultivars) grown in France allowed to identify 36 alleles at seven loci: Glu-A1, Glu-B1, Glu-R1, Gli-R2, Glu-B2, Glu-A3 and Glu-B3. Glu-B1 and Glu-B3 loci were the most polymorphic with 9 alleles each. On the basis of allelic frequencies at the seven loci, genetic distances between hexaploid triticales grouped according to their origins revealed two groups: winter triticales mostly originating from European germplasm and spring triticales essentially of CIMMYT origin. Comparison of allele frequencies between hexaploid triticale cultivars and a world collection of bread (Triticum aestivum) and durum (Triticum durum) wheat was investigated at Glu-A1 and Glu-B1: only a significant association was found for Glu-A1 alleles (?2 = 2.26, p =0.36) between triticale and bread wheat