Albrechtsen, Hans-Jørgen

Dechesne, Arnaud

Hedegaard, Mathilde Jørgensen

Papadopoulou, Aikaterini

Smets, Barth F.

Online Research Database In Technology

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Downloaded from orbit.dtu.dk on: Dec 20, 2017Methanotrophs assisted bentazone degradationPapadopoulou, Aikaterini; Hedegaard, Mathilde Jørgensen; Dechesne, Arnaud; Albrechtsen, Hans-Jørgen; Smets, Barth F.Publication date:2014Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Papadopoulou, A., Hedegaard, M. J., Dechesne, A., Albrechtsen, H-J., & Smets, B. F. (2014). Methanotrophsassisted bentazone degradation. Poster session presented at International Biodegradation and BiodeteriorationSymposium  , Lodz, Poland.Metha ot o h  a i ted n r p s ss sAik t i i P d l  M thild  H d d Ja er n apa opou ou, a e e egaar - ørgensen& B th arTechnical University of Denmark, Department of Environmental Engineering, Makap@env.dtu.dkIntroductionDrinking water is increasingly threatened by contamination frompesticides and pesticide metabolites including bentazone a thiadiazine, ,h bi id i t t i d t A bi d t fter c e pers s en n groun wa er. naero c groun wa er o encontains methane, which is easily oxidized by methane-oxidizingbacteria (MOB) upon groundwater aeration These bacteria have known.t b li d d ti ti i t l f icome a o c egra a on proper es aga ns some c ass o organ ccontaminants.Goal: Test whether MOBs enriched from rapid sand filters cancometabolically degrade bentazone.Bentazone Removal & Mineralization100• No effect of CH4 in removal )kinetics(%).4 C0 (50C/1414C• Higher total mineralization in theabsence of CH40 1 2 3 4050 100 150 200Time (hours)14C i li id h (1 5 /L CH )  n qu  p ase .  mg  414C i li id h (0 /L CH ) n qu  p ase  mg  4Autoclaved control14CO (1 5 /L CH )2 ,  mg  414CO2 (0 mg/L CH4)14CO2 in autoclaved controlsCH4 effect – Enzyme competition• CH concentration maintained at 5 mg/L4 • r=removal rate 30 • At low bentazone 20H4 t ti  th  10CH4 concen ra ons, eth C effect of methane is 5r witnot significant/ rwH4/ • The higher the 0CHbentazone out tho concentration, the -5r withigher the ratio of rremoval without 2 0 0 0 0 0 CH / ith  CH0-0.2-1. 0-10-30-5 20 4w 40. 1. 1 3 100-Bentazone concentration (g/L)  )g/L)200ugal (ova150moReme R100onazo• Effect of methane entobserved in high CH50Be4 ve (>0.5 mg/L) 0ativconcentrations ula0 1 2 3 4 5 20 40 60umTime (days)Cu Bentazone Concentration Methane ConcentrationNo CH45 mg/L CH4200 ug/L     No CH4200 ug/L  0.5 mg/L CH4 be ta o e de adation z n gr n A d D h  H J  Alb ht  , rnau ec esne, ans- ørgen rec sen,F  S t. me siljøvej, Building 115, 2800 Kgs Lyngby, DenmarkMaterials and Methods• Material from rapid sand filters used to enrich th t hi  lt  i  ti  fl  l b l  me ano rop c cu ure n con nuous - ow a sca ereactorsB h  f b  l & i li i• atc assay o entazone remova m nera zat on14C carbonyl labeled bentazone in concentrations • -ranging from 0.2 to 2000 ug/L with and without methane (triplicates)• Abiotic controls (autoclaved Filtralite)M th  l i  GC FID• e ane ana ys s – -Concentration Effect1 5 /L).) (ug/g/L) 150val (al (ugmov1.0movaRemRem 100ne Rne Rzon0.5azonntazenta50Ben0.4ve Bve Bativativ0.2mul 0mulCum0 2 4 6 10 20 30 40Cum0.0Ti (d )C0 2 4 10 15 20 25 30 35 40 45 50Time (days) B t C t ti M th C t time aysBentazone Concentration Methane Concentration N CH4en azone oncen ra on e ane oncen ra onNo CH4  5 mg/L CH40 2 ug/Lo 5 mg/L CH420 ug/L No CH4  .  0 5 ug/L 5 mg/L CH4No CH450 ug/L  5 mg/L CH4 N CH4.     5 /L CH4 No CH4200 ug/L 5 mg/L CH4o 2.0 ug/L   mg       30(%)• Removal in all 20/C0 concentration rangesCO2/d14C• Slower removal rate in atedthe presence of CHmula410cumDelay observed at high inal •Fi CH4 concentrations00.2 0.2 0.5 0.5 2.0 2.0 20 20 50 50 20020020020050050020002000Microcosms with 5 mg/L methaneMicrocosms with 0 5 mg/L methane  .   Microcosms without methaneConclusions• Microbial communtiy fed only with methane in drinking water for > 1year shows efficient and stable bentazone degradation in allconcentration rangesTwo possible removal patterns: (a) not inhibited by methane at low•b (b) h h b d h hentazone concentrations, met ane-in i ite at igconcentrations• Not consistent pattern with the typical cometabolic process• Methane’s presence slows down bentazone degradation process unlesspresent in low concentrations (<10 μg/L)• Observed competitive inhibition between methane and bentazone

Methanotrophs assisted bentazone degradation

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Methanotrophs assisted bentazone degradation

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