Evaluation of Antibiofilm Activity of Dentol

Abstract

Biofilm is a community of bacteria which are attaching to a surface. They are responsible for many of catheter related or dental plaque related infectious diseases. Different approaches have been used for preventing biofilm related infections in health care settings. Many of these methods have disadvantages such as chemical based complications, and emergent antibiotic resistant strains. Therefore antibiofilm effect of Dentol was evaluated on two important medical bacterial biofilms in this study. Methods: Minimum inhibitory concentrations (MIC) of Dentol was calculated by serial dilution method by microtiter plates against standard bacteria. Antibiofilm effect of Dentol was evaluated by microtiter plate assay and after measuring absorbance in 550 nm, the difference between groups was calculated by Cruskal-wallis and Mann-withney tests. Results: 1:80 (12.5 μl/ml) & 1:20 (50 μl/ml) dilutions of commercially available Dentol were measured as MICs for S.aureus and P.aeruginosa, respectively. Two-fold dilutions of Dentol MICs completely eradicated and inhibited biofilm formation. Statistical analysis between test groups revealed significant differences. Disscussion: Dentol is a medicinal herbal essential oil derived from Satureja khuzestanica (SKEO). Several in vitro studies have reported its antidiabetic, analgesic, anti-inflammatory, and anti-hyperlipidemic effects. So because of complications that have been encountered in health care settings from routine methods of catheter processing, and biofilm related problems, efficacy of Dentol for destruction of bacterial biofilms as a safe material was studied