Bosco, Filippo

Denne afhandling repræsenterer en del af kravet for at opnå Ph.D. graden ved DTU Nanotech. Projektet er udført på Danmarks Tekniske Universitet, Danmark (DTU) ved Institut for Micro og Nanoteknologi i perioden fra november 2008 til oktober 2011. Projektet er en del af X-sense forskningsnetværk, finansieret af det Danske strategiske Forskningsråd. Projektet er vejledt af Professor Anja Boisen.Målet med projektet er at designe og fabrikere en kompakt og billig måleenhed til detektion af eksplosive stoffer i luft og væske. Målet er at integrere fire forskellige detektions principper (Cantilever overflade stress, kalorimetrisk, kemisk farveskift og RAMAN) i en enkelt bærbar platform. Mit Ph.D. projekt har primært fokuseret på udviklingen af Cantilever overflade stress sensoren. Sekundært beskrives designet og produktionen af den centrale sensor platform hvori de fire detektions principper skal integreres. Denne platform er udviklet med fokus på Cantilever sensoren. De indledende test af RAMAN sensoren er medtaget. Den kalorimetriske og kemisk farveskift sensor er stadig under udvikling og derfor ikke medtaget.Denne afhandling beskriver en High-Throughput Label-free sensor som udnytter overflade stress skift af en Cantilever sensor. Disse sensorer er ofte blevet tillagt evnen til at kunne udføre multiple parallelle målinger, men der er endnu ikke bygget et system der kan producere en så stor simultan datamængde med et simpelt fluid system. I dette system bruges optik og mekanikken fra en DVD disk aflæser til håndtering af de fluide prøver og til aflæsning af Cantilever afbøjning og resonans frekvens. Systemet muliggør en simultan parallel måling af fluide prøver ved brug af mange hundrede individuelle Cantilever i løbet af få minutter.Tre separate generationer af systemer er udviklet og testet i løbet af projektets tre år. Hvert system er brugt til at detektere proteiner, antistoffer og eksplosive stoffer. Diverse rotations platforme til gas og fluide målinger er desuden udviklet, produceret og testet. Den nuværende platform repræsenter et nyt og effektiv værktøj til at analysere biokemiske reaktioner ved hjælp af en Cantilever sensor, hvor målesikkerheden er valideret gennem statistisk analyse og gennem den simultane karakterisering af hver Cantilever.This thesis has been written as a partial fulfillment of the requirements for obtaining the PhD degree at DTU Nanotech. The project has been carried out at the Technical University of Denmark (DTU) at the department of Micro- and Nanotechnology, in the three-years period between November 2008 and October 2011. The project was part of the Xsense research network, funded by the Strategic Danish Research Council, and supervised by Prof. Anja Boisen.The goal of the Xsense project is to design and fabricate a compact and cheap device for explosive sensing in air and liquid. Four different technologies (Cantilevers, Calorimetric, Colorimetric and Raman) were to be integrated into a single portable platform. My PhD project has been focusing on the cantilever technology part. Furthermore, I have been addressing the issue of designing and fabricating the overall sensing platform, which is going to integrate the four different sensors. The platform was developed specifically for cantilever sensor applications Preliminary tests on Raman-based device integration has been carried out. The inclusion of the other two sensing techniques is currently under development.This thesis reports on the demonstration of a high-throughput label-free sensor platform utilizing cantilever based sensors. These sensors have often been acclaimed to facilitate highly parallelized operation. Unfortunately, so far no concept has been presented which offers large data sets as well as easy liquid sample handling. We use optics and mechanics from a DVD player to handle liquid samples and to read-out cantilever deflection and resonant frequency. In a few minutes, several liquid samples can be analyzed in parallel, measuring over several hundreds of individual cantilevers.Three generations of systems have been developed and tested during the three years. These devices have been used for sensing of proteins, antibodies and explosives. Different rotating platforms, specifically designed for gas and liquid measurements, have also been designed, fabricated and tested. The resulting platform represents a completely novel and powerful tool for analyzing biochemical reactions through cantilever sensors, where the reliability of the measurements is ensured by statistical analysis and by parallel characterization of cantilevers

Online Research Database In Technology

A DVD-ROM based high-throughput cantilever sensing platform

http://orbit.dtu.dk/ws/files/51617200/Thesis_Bosco_28_10_2011.pdf

A DVD-ROM based high-throughput cantilever sensing platform

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Online Research Database In Technology