Boye, Mette

Dalsgaard, Inger

Ingerslev, Hans-Christian

Madsen, Lone

Online Research Database In Technology

                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Dec 20, 2017The gut microbiota in rainbow trout changes after first feeding and is dependent on thetype of dietIngerslev, Hans-Christian; Boye, Mette; Dalsgaard, Inger; Madsen, LonePublication date:2012Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Ingerslev, H-C., Boye, M., Dalsgaard, I., & Madsen, L. (2012). The gut microbiota in rainbow trout changes afterfirst feeding and is dependent on the type of diet. Poster session presented at 14th International Symposium onMicrobial Ecology, Copenhagen, Denmark.The gut microbiota in rainbow trout changes after first feeding and is dependent on the type of dietH.-C. Ingerslev, M. Boye, I. Dalsgaard and L. MadsenNational Veterinary Institute, Technical University of Denmark, Bülowsvej 27, DK-1870 Frederiksberg C, Denmark. E-mail: hain@vet.dtu.dkINTRODUCTIONIn the very first life stage of most fish species nutrition only comes from a yolk sac originating from the egg. During this larval phase the intestine is being formed and becomes func-tional as the amount of yolk sac protein is used up and feeding from external sources then slowly takes over. Thus, this devel-opmental window is a good model to study the influence of fee-ding on the gut microbiota dynamics. Further, the origin of the feed sources may also influence the microbial community. In this study we examined this in the commercially produced species rainbow trout (Oncorhynchus mykiss) using next-generation sequencing. 0% 20% 40% 60% 80% 100%BacteriodetesProteobacteriaFirmicutesActinobacteriaCyanobacteriaSpirochaetesPlanctomycetesTenericutesFusobacteriaVerrumicrobiaSAR406AcidobacteriaTM6TM7ChlamydiaeOthersMATERIALS & METHODSIntestines from experimental fish were collected for analysis 1 day before first feeding (n=31), 26 (n=40) and 49 (n=43) days post first feeding. Mean size of sampled fish was 0.12 g, 0.31 g and 1.08 g at the three sampling points, respectively. The fish collected at days 26 and 49 had been fed a) commercial diet of marine origin (Inicio, Biomar A/S) or b) tailormade diet containing plant parts; rapeseed oil (instead of fish oil as in Inicio) plus pea proteins. DNA was extracted from the intestines and subsequently used for PCR by universal barcoded primers targeting the V5 region of the 16S rDNA gene. Sequencing was conducted on the Illumina HiSeq 2000 platform. Obtained sequences were sorted and normalised by BION-meta software1. Taxonomic classification was accor-ding to the Greengene database. OBJECTIVESi) To examine the microbiota in the intestine of rainbow trout before first feeding. ii) To study the impact on the intestinal microbiota after giving feed to the fish of either marine or plant origin.0% 20% 40% 60% 80% 100%MarinePlantTop 10 phylaTop 10 phyla0% 20% 40% 60% 80% 100%MarinePlantTop 10 phylaTop 10 phylaTop 10 phylaTop 10 generaTop 10 generaTop 10 genera1 day before first feeding26 days post first feeding49 days post first feedingCONCLUSIONSi) The intestinal community changed from a Bacteriodetes dominated towards a Proteo-bacteria and Firmicutes dominated microbiota in connection to first feeding. The bacterial flora before first feeding may reflect the one present in the surrounding water. ii) Fish fed a plant based diet had a higher ratio of Firmicutes relative to marine fed fish. Among these, there was a higher amount of the genera Streptococcus, Leuconostoc and Weissella in fish fed a plant based diet com-pared to a marine diet.   Larvae FryExternal food intake initiates during the metamorphosis from larvae to fry when yolk sac is depletedREFERENCES1. Larsen N., Ingerslev H.-C., Molbak L., Ahrens P. and Boye M. ftp://genomics.dk/pub/BION. BION-meta, a 16S/23S sequence classificationpipeline. In preparation.a       b      c       d       e       f       g       h       i        j   This project was funded by The Danish AgriFish Agency, Ministry of Food, Agriculture and Fisheries. The project (OPTIFISH) is a part of the research programme Organic RDD, which is coordinated by ICROFS (Project ID: 3405-10-OP-000128).   

The gut microbiota in rainbow trout changes after first feeding and is dependent on the type of diet

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The gut microbiota in rainbow trout changes after first feeding and is dependent on the type of diet

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